摘要
目的观察Ｒｂ１对局灶性脑缺血区ＳＯＤ、ＮＯ含量的影响。方法ｗｉｓｔａｒ大鼠３０只体重３００－３５０ｇ，雌雄兼用，随机分为３组（ｎ＝１０）：①正常对照组（ＳＯ）；②大脑中动脉梗塞对照组（ＭＣＡＯ）；③药物组：２０μｇ／ｋｇ－１Ｒｂ１连续腹腔注射１０天。①②组生理盐水１０ｍｌ／ｋｇ－１连续腹腔注射１０天。于第１０天灌胃后３０ｍｉｎ①组动物处死取脑制成匀浆，②③组行ＭＣＡＯ后３０ｍｉｎ，处死取脑制成脑匀浆。根据ＮＯ与氧生成硝酸盐、亚硝酸盐，硝酸盐与磺酸及奈乙烯二胺酸盐反应为粉红偶氮化合物及酶联免疫吸附实验原理，采用比色法分别测定脑匀浆ＮＯ、ＳＯＤ含量。结果ＳＯ组脑匀浆ＮＯ含量为７．４１μｍｏｌ·ｍｇ－１（蛋白），ＳＯＤ为００２１μｍｇ·ｍｇ－１（蛋白）；ＭＣＡＯ组脑匀浆ＮＯ含量为１４７３μｍｏｌ·ｍｇ－１（蛋白），ＳＯＤ含量为００１９３ｍｇ·ｍｇ－１（蛋白）；Ｒｂ１组ＮＯ为７３６μｍｏｌ·ｍｇ－１（蛋白），ＳＯＤ含量为００２１９ｍｇ·ｍｇ－１（蛋白），各组间比较有显著差异（Ｐ＜０．０１）。结论①Ｒｂ１能降低ＭＣＡＯ区ＮＯ含量，降低ＮＯ含量的原理可能与ＳＯＤ有关。②Ｒｂ１能增加缺血脑组织ＳＯＤ含量。③脑中?
Objective:To observe the effect of Rb1 on SOD and NO contents in cerebral tissue
with MCAO Methods:30 Wistar rats (weight 300350g;both male and female) were divided into 3
groups randomly and were administered continuously by 10 mlkg-1 of normal saline in group 1
and group 2 and 20 gkg-1Rb1 in group 3 for 10 days.Then the contents of No and SOD were
detected by colorimetric method in cerebral hemogenate obtained by removing and mixing rats'
brains following killling them 30 minutes after intragastric perfusion.Results:The contents of
SOD in SO,MCAO and Rb1-treated groups were respectively 0.021,0.0193 and 0.0219 mgkg-1
protein and the conversations of NO in these 3 groups were respectively 7.410,14.73 and
7.36molmg-1 protein.The differences between the 3 groups were significant
(P<0.01).Conclusion:Rb1 can significantly reduce the contents of NO in cerebral tissue with
MCAO.The conversations of SOD in MCAO region are obviously increased.And the changes in
contents of NO and SOD have a positive correlation.
出处
《泰山医学院学报》
CAS
1999年第2期111-113,共3页
Journal of Taishan Medical College
关键词
大脑中动脉梗塞
一氧化氮
SOD
RB1
脑组织
middle cerebral artery
occlusion(MCAO)
nitric oxide (NO)
superoxide dismutase (SOD)
Rb1 cerebral hemogenate
