摘要
目的 评价铝盐佐剂对甲型肝炎病毒抗原 (HAAg)和乙型肝炎病毒表面抗原 (HBsAg)细胞免疫反应的影响。方法 以甲型肝炎病毒灭活抗原和乙型肝炎病毒基因工程表达抗原为对象 ,采用小鼠迟发型超敏反应 (DTH)、脾脏淋巴细胞增殖实验和淋巴细胞亚型分群实验 ,检测铝盐佐剂吸附前后的细胞免疫反应变化 ,并进行统计学分析。结果 甲型肝炎病毒抗原佐剂吸附后 ,DTH反应强度和特异性T淋巴细胞增殖强度有所降低 ,但无统计学意义 (P >0 0 5 ) ;乙型肝炎病毒表面抗原吸附佐剂后 ,特异性T淋巴细胞增殖显著降低 (P <0 0 5 ) ,CD4+ /CD8+ 比值明显升高(P <0 0 5 )。结论 铝盐佐剂可减弱杀伤型T细胞的分化和增殖 ,优势调节T淋巴细胞向辅助型T细胞 (TH)分化 。
Objective To assess the cellular immunity effects of aluminium salt to HAAg and HBsAg Methods We detected the immunoreaction degree which induced by HAAg、HAAg+Al、HBsAg and HBsAg+Al by proliferation activity test of mice splenocyte、DTH response、determination of lymphocyte subpopulation and analyzed by statistics Results When Hepatitis A antigen was absorbed to aluminium salt,the reaction degree of DTH and the proliferation of specificity T lymphocyte was decline,but have not significantly decline( P >0 05),while after hepatitis B surface antigen was absorbed,the proliferation of specificity T lymphocyte was significantly decline( P <0 05)and the rate of CD4 +/CD8 + was significantly rise( P <0 05) Conclusions Alum adjuvant could reduce the difference and proliferation of cytotoxic T lymphocyte,mainly induce difference to helper T lymphocyte,and some kinds reduce the ability of eliminating microbial pathogens in cells
出处
《中国预防医学杂志》
CAS
2003年第1期26-29,共4页
Chinese Preventive Medicine
