摘要
目的 :拟在白噪声下观察对大鼠学习记忆的影响及海马区NOS的变化 ,探讨噪声所致大鼠学习记忆下降的机制。方法 :44只SD大鼠 ,其中 2 0只随机分两组 ,分别在持续 80dB (A)白噪声和无噪声的环境下 ,在Y -迷宫中进行空间辨别学习记忆训练 ,了解噪声对大鼠学习记忆的影响 ,另 2 4只大鼠随机分三组 :噪声训练组、正常训练组和噪声观察组 ,以同样的方法训练 ,用免疫组化的方法观察持续噪声对海马区NOS阳性神经元表达变化。结果 :噪声能降低大鼠学习记忆能力 ,而且海马区NOS阳性神经元较正常对照组的数量及染色强度显著降低。结论 :噪声降低海马区神经元活性 ,NOS的合成减少 ,抑制海马习得性长时程突触增强 ,影响记忆的获得与保持 。
Objective: To observe the effects of exposure to white noise on the procession of learning and memory, and the expression of NOS (nitric oxide synthase) in hippocampus of rats Method: 20 rats were randomly divided into two groups for observation of spatial learning and memory, one group exposed to 80 dB white noise during Y-maze training, the other group received Y-maze training under normal condition Another 24 rats were randomly divided into 3 groups, they experienced normal Y-maze training, noise Y-maze training and sole noise as control respectively Immunohistochemical assay was used to investigate the expression of NOS positive neurons Result: Noise hampered the ability of rats' spatial learning and memory, furthermore, the number of NOS positive neurons in hippocampus of noise training group was less than that of normal training group Conclusion: The activity of NOS positive neuron in hippocampus was decreased by exposure to noise, suggesting the inhibition of learning dependent long term procession in hippocampus and corresponding difficulty in obtaining and maintaining of memory
出处
《中国心理卫生杂志》
CSSCI
CSCD
北大核心
2003年第2期80-83,共4页
Chinese Mental Health Journal
基金
全军十五指令性课题 (0 1L0 71 2 )
关键词
噪声
大鼠
学习记忆
海马区
神经元
一氧化氮
一氧化氮合酶
psychiatry stress animal study noise learning and memory nitric oxide synthase rat hippocampus
