摘要
目的 :探讨决明子降血脂作用机理。方法 :采用体外大鼠肝细胞培养 ,以Folin -酚试剂法测定肝细胞蛋白质含量 ,并以液体闪烁计数法测定肝细胞中合成 14C -胆固醇的量。结果 :决明子浸膏剂对 14C -胆固醇的合成有一定的阻抑作用 ,而决明子苷B未出现明显的抑制合成 14C -胆固醇的作用。结论 :决明子浸膏剂降血脂作用是在一定程度上抑制了胆固醇的合成 ,而决明子苷B降血脂的主要途径不是抑制胆固醇的合成。
OBJECTIVE:To study the mechanism of Cassia obtusifolia L in decreasing blood-lipid.METHODS:Primary cultures of rat hepatocytes in vitro were used to determine the concentrations of proteins in hepatocytes by the method of Folin-phenol reagent and to assay the cholesterol synthesis from 14 C-acetate with liquid scintillation count.RESULTS:Exˉtract of Cassia obtusifolia L inhibited cholesterol synthesis from 14 C-acetate,but cassiaside B didn't affect cholesterol syntheˉsis.CONCLUSION:The mechanism of extract of Cassia obtusifolia L in decreasing blood-lipid may result from inhibition of cholesterol synthesis,but inhibiting cholesterol synthesis may be not the main approach of cassiaside B in decreasing blood-lipid.
出处
《中国药房》
CAS
CSCD
2003年第4期202-204,共3页
China Pharmacy
基金
重庆市科委基金资助项目 (1998-28-172)
关键词
决明子
降血脂作用
肝细胞培养
液体闪烁计数
实验研究
Cassia obtusifolia
hepatocyte culture
liquid scintillation count
decreasing blood-lipid action
