摘要
目的 探讨前列腺素E1 (PGE1 )在脑缺血再灌注损伤中对脑组织过氧化的影响、对脑细胞膜ATP酶的保护、对血浆炎性细胞因子及内皮素的干预作用。方法 采用线栓法阻断大鼠左侧大脑中动脉 ,造成局灶性脑缺血再灌注模型 ,用药组于缺血前 5minivgttPGE1 (1 2 ,2 4 ,48μg·kg- 1 ) ,模型组及假手术组给予等容积NS。各组在大脑中动脉阻塞(MCAO) 60min ,再灌注 60min后 ,断头、取脑匀浆 ,测定其丙二醛 (MDA)含量 ,总超氧化物歧化酶 (SOD)活性 ,谷胱甘肽过氧化物酶 (GSH Px)活性及Na+ ,K+ ATP酶 ,Ca2 + ATP酶 ,Mg2 + ATP酶活性。心脏取血分离血浆测定其肿瘤坏死因子 (TNFα)的浓度及白介素 (IL 1 β、IL 6)和血浆内皮素 (ET 1 )的含量。结果 缺血再灌注模型组与假手术组相比 ,MDA含量升高 (P <0 0 5)、SOD及GSH Px活力降低 (P<0 0 5) ,Na+ ,K+ ATP酶、Ca2 + ATP酶、Mg2 + ATP酶活性降低 (P <0 0 5) ,TNFα、IL 1 β、ET 1含量升高 (P <0 0 5)。PGE1 3剂量组与模型组相比 ,MDA含量降低 (P <0 0 5) ,SOD及GSH Px活力升高 (P <0 0 5) ,Na+ ,K+ ATP酶、Ca2 + ATP酶、Mg2 + ATP酶活性升高 (P <0 0 5 ) ,Mg2 + ATP酶活性亦升高 ,TNFα、IL 1 β、ET 1含量降低 (P<0 0 5)。结论 PGE1 对缺血再灌注?
AIM To investigate the protective effects of prostaglandin E 1(PGE 1) against peroxidation and on the membrane ATPase cellular inflammation factor and endothelin in cerebral ischemia reperfusion injury in rats. METHODS Cerebral ischemia reperfusion was induced by 60 minutes of middle cerebral artery occlusion followed by 60 minutes of reperfusion.Three doses of PGE 1(12,24 and 48 μg·kg -1 ) was injected intravenously 5 minutes before the occlusion,equal volume of normal saline was given in model and sham operation groups.Then the brains were assayed for content of MDA,and activities of SOD,GSH Px,Na +,K + ATPase,Ca 2+ ATPase and Mg 2+ ATPase.The plasma was assayed for TNFα,IL 1β,IL 6 and ET 1. RESULTS The contents of MDA TNFα?IL 1β and ET 1 in model were increased compared with sham( P <0 05) while the activities of SOD,GSH Px,Na +,K + ATPase,Ca 2+ ATPase and Mg 2+ ATPase were significantly decreased compared with model group,all three doses of PGE 1 lowered the contents of MDA, TNFα?IL lβ and ET 1, increased the activities of SOD,GSH Px,Na +,K + ATPase,Ca 2+ ATPase and Mg 2+ ATPase( P <0 05). CONCLUSION The protective effect of PGE 1 against cerebral ischemia reperfusion injury has relation to the inhibition of peroxidation, the protection of activities of SOD,GSH Px,Na +,K + ATPase,Ca 2+ ATPase and Mg 2+ ATPase,and the lowering of the contents of cytorines and ET 1.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第4期427-431,共5页
Chinese Pharmacological Bulletin
基金
山东科技厅自然科学基金资助课题No013130 114
