摘要
目的:观察前列地尔在大鼠血管球囊损伤术后对血浆内皮素(ET)及炎性细胞因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平的影响。方法:采用大鼠腹主动脉球囊损伤模型,用药组术前连续5d经尾静脉给予前列地尔(8,24,72μg·kg-1),模型组及假手术组给予等容积生理盐水。各组动物于术后6,24h,10,21d4个时间点取血,采用放射免疫方法测定血浆中ET及血清中IL-1β,IL-6和TNF-α的含量。结果:前列地尔各组24h血浆ET含量[(34±s4),(34.9±1.7),(29±3)ng·L-1]与模型组[(73±8)ng·L-1]相比显著降低,P<0.01。前列地尔各组均可在高峰时显著降低IL-1β[(0.267±0.012),(0.136±0.017),(0.142±0.015)μg·L-1,P<0.01],IL-6[(340±43),(243±20),(199±22)μg·L-1,P<0.01]和TNF-α[(3.34±0.24),(1.73±0.27)(1.66±0.29)μg·L-1,P<0.01]的含量,并呈良好的剂量相关性(r=0.747,0.907,0.747)。结论:前列地尔降低大鼠血管球囊损伤术后血浆中ET及血清中IL-1β,IL-6,TNF-α水平的作用,是其干预血管再狭窄形成的重要机制。
AIM： To evaluate the effects of alprostadil preventing restenosis after balloon injury and its mechanisms in rats. METHODS： Rats were divided into five groups. Three alprostadil dose （8, 24, 72 μg · kg^-1 ） were injected respectively via tail vein 5 d before operation, equal quantity of normal saline were injected in model group and sham operation group. Rat＇s abdominal aorta was damaged by balloon catheter in the first four group. ET, IL-1β, IL-6,TNF-α were tested at 6, 24 h, 10 and 21 d after operation. RESULTS ： Compare with model group （ （73 ± s8） ng · L^-1）, alprostadil （8,24,72 μg · kg^-1） could significantly decrease the plasma levels of ET （（34±4）,（34.9±1.7）, （29±3） ng·L^-1） in 24 h （P 〈0.01）. Alprostadil （8,24,72μg ·kg^-1） could significantly decrease the serum levels of IL-1β （（0.267±0.012）, （0. 136 ±0.017）, （0.142 ± 0.015）βg·L^-1,p〈0.01）, IL-6（（340±43）, （243 ±20）, （199 ±22）μg· L^-1, P〈0.01）, TNF- α（（3.34±0.24）, （1.73±0.27）,（1.66±0.29） μg·L^-1 ,P 〈 0.01 ）, and revealed a dose-dependent （r = 0.747,0. 907,0. 747 ）. CONCLUSION ： The reduction of the plasma levels of ET and the serum levels of IL-1α, IL-6 and TNF-α plays an important role in the protective mechanisms against vascular restenosis of alprostadil.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2005年第9期719-722,共4页
Chinese Journal of New Drugs and Clinical Remedies
