摘要
目的 :从孕妇血浆中提取胎儿DNA ,寻找一种非创伤性产前诊断的新方法。方法 :采用煮沸法从血浆中提取DNA ,结合巢式聚合酶链反应技术对 4 0例 10～ 4 0孕周的孕妇血浆中的胎儿DNA进行特异性扩增 ,扩增的基因为Y染色体的重复序列 (DYZ1)基因 ,扩增片段大小为 10 2bp。结果 :30例孕育男性胎儿孕妇中有 2 6例血浆中出现DYZ1基因扩增带。 10例孕育女性胎儿孕妇血浆中 ,仅 1例出现假阳性结果。结论 :采用巢式聚合酶链技术在不同的孕期检测母体血浆中的胎儿DNA均有较高的灵敏度和特异性 ,作为一种非创伤性产前诊断的方法应用于临床具有可行性。
Objective: To search for a new method of non invasive prenatal gene diagnosis. Methods: The DNA was extracted from maternal plasma using a rapid boiling method. A human DYZ1 gene of Y chromosome of fetal DNA sequence was amplified by nested polymerase chain reaction(PCR) from forty pregnant maternal plasma(10～40 weeks).The 102 bp specific fragment was obtained. Results: The fragment was identified in 26 of 30 male bearing pregnant women plasma. The diagnostic accordance rate was 87%(26/30). Only one of the other 10 female bearing pregnant women had positive result. Conclusion: The high sensitivity and specificity with which the plasma polymerase chain reaction based method can be performed makes it an apromising method for non invasive prenatal gene diagnosis.
出处
《武汉大学学报（医学版）》
CAS
2003年第2期192-194,共3页
Medical Journal of Wuhan University
关键词
产前诊断
聚合酶链反应
胎儿DNA
prenatal diagnosis
polymerase chain reaction
fetal DNA
