摘要
目的：探讨利用O型血孕妇血浆中游离胎儿DNA检测胎儿ABO血型的可行性。方法：用酚／氯仿法从39例O型血孕妇（孕36～40^＋3周）且孕男性胎儿的血浆中提取胎儿DNA，巢式PCR扩增血浆中的SRY基因证实胎儿DNA存在，再用特异性序列-聚合酶链反应（SSP-PCR）检测胎儿ABO血型。用常规血清学方法测母体及新生儿血型。结果：10例孕A型血胎儿的孕妇血浆中扩增出A基因8例，6例孕B型血胎儿的孕妇血浆中扩增出B基因6例，23例孕O型血胎儿扩增出O基因而无A或B基因21例，准确性和敏感性分别为89．7％（35／39）和87．5％（14／16）。结论：利用孕妇外周血中游离胎儿DNA检测胎儿ABO血型可行，对诊断和预防新生儿ABO血型不合性溶血病的发生具有积极意义。
Objective：To explore the feasibility of prenatal diagnosis of fetal ABO blood types with fetal DNA in maternal plasma of O blood types. Methods：Peripheral blood samples were obtained from 39 pregnant women whose blood types were O（pregnancy 36-40＋3weeks）. DNA from maternal plasma was extracted by phenol/chloroform from plasma. The existence of fetal DNA was confirmed by amplified fetal SRY gene. Fetal ABO genotype was detected by SSP-PCR. Routine serological method was used to test ABO phenotype of mothers and newborns. Results：Among the 39 specimens,10 A gen- otype were detected in 8 cases,6 B genotypes were detected in 6 cases,and 23 O genotypes were detected in 21 cases. The accuracy and sensitivity were 89.7% （35/39） and 87.5% （14/16）. Conclusion：It is feasible to detect fetal ABO blood types with fetal DNA in maternal plasma,and it is of significance to diagnose and prevent hemolytic disease of newborns.
出处
《现代妇产科进展》
CSCD
北大核心
2007年第12期917-919,共3页
Progress in Obstetrics and Gynecology
