摘要
目的探讨用孕妇血浆中游离DNA检测胎儿ABO血型方法的可行性。方法应用DNA提取试剂盒(qiagen德国)从46例9～22周孕妇血浆提取胎儿DNA,采用复合PCR-RFLP检测胎儿ABO血型,并用常规血清学方法检测母体及新生儿血型。结果 46例样本中,产前检查能正确显示胎儿血型的有38例,总检出率为(38/46)82.61%。其中:①孕妇血型为O型,丈夫血型为A型16例,产前检测孕妇胎儿血型为A型,待婴儿出生后血清学检测出12例,符合率为(12/16)75.00%;②孕妇血型为O型,丈夫血型为B型占13例。产前检测孕妇胎儿血型为B型,待婴儿出生后血清学检测出9例,符合率为(9/13)69.23%。③孕妇血型为O型,丈夫血型为O型占11例,产前检测孕妇胎儿血型为O型,待婴儿出生后血清学检测出10例,符合率为90.90%。总检出符合率(10/11)81.58%。结论用孕妇血浆中游离DNA检测胎儿ABO血型方法可行,对诊断和预防新生儿ABO血型不合性溶血病具有积极意义。
Objective：This study aimed to evaluate the applicability of PCR-RFLP based method in prenatal ABO genotyping.Methods：Plasma samples from 46 pregnant women（at 9 to 22 gestations） were studied for prenatal ABO genotyping.Cell-free fetal DNA was extracted from maternal plasma followed by PCR amplification and RFLP analysis.For quality control,serologic ABO phenotyping for both newborns and mothers was performed.Results：Among 46 testing samples,ABO genotyping with cell-free fetal DNA correctly indicated the serologic type in 38 samples with a high accuracy of 82.61%.Based on the ABO genotypes of the husband,the 46 samples were further divided into 3 subgroups named A,B and O,as all the pregnant women enrolled were O-group.Of 16 samples from pregnant women with A-group husbands,there were 13 correct results.Likewise,the accuracy could reach 78.57%（11 /14） and 87.5 %（14 /16） for group B and group O,respectively.The genotype results were subsequently evaluated using serologic tests for newborn ABO phenotyping.Conclusion：The PCR-RFLP based prenatal ABO genotyping with cell-free fetal DNA is feasible and should become common practice in the near future for management of newborn hemolytic diseases.
出处
《中国优生与遗传杂志》
2010年第8期51-52,共2页
Chinese Journal of Birth Health & Heredity
基金
陕西省卫生厅卫生科研基金项目(项目编号08D08)
