摘要
目的 探讨MMP 2、TIMP 2、MT1 MMP与成釉细胞瘤局部侵袭特性的生物学关系。方法 采用RT PCR方法检测基质金属蛋白酶及其抑制剂和激活剂MMP 2、TIMP 2、MT1 MMP在成釉细胞瘤的表达及含量。结果 在成釉细胞瘤组织中 ,MMP 2、MT1 MMP、TIMP 2的mRNA均表达 ,且表达水平均显著高于正常牙囊组织 ,复发及实性成釉细胞瘤的TIMP 2、MT1 MMP相对含量均显著高于原发和囊性成釉细胞瘤。结论 在成釉细胞瘤组织中 ,MMP 2、MT1 MMP、TIMP 2转录水平的增高可能与其侵袭特性有关 ,MT1 MMP/TIMP 2表达水平可能与其侵袭能力有关。
Objective To investigate the biological mechanism between invasion and MMP 2、TIMP 2、MT1 MMP in ameloblastoma. Methods Detect the expression levels of matrix metalloproteinase 2 (MMP 2), tissue inhibitor of metalloproteinase 2 (TIMP 2) and membrane type 1 matrix metalloproteinase (MT1 MMP) in ameloblastoma using RT PCR. Results All investigated ameloblastoma expressed genes encoding mRNA for MMP 2, MT1 MMP and TIMP 2. The expression levels of MMP 2, MT1 MMP and TIMP 2 mRNA in ameloblastoma were significantly higher than those in dental germ, and the mRNA levels of TIMP 2 and MT1 MMP in recurrent and solid ameloblastoma were significantly higher than those in primary and cystic ameloblastoma respectively. Conclusion The elevated levels in MMP 2, TIMP 2 and MT1 MMP transcription may contribute to the invasion of ameloblastoma, whereas the invasive capacity in ameloblastoma is more likely related to the expression levels of TIMP 2 and MT1 MMP.
出处
《口腔颌面外科杂志》
CAS
2003年第1期21-24,共4页
Journal of Oral and Maxillofacial Surgery
