摘要
目的探讨RECK和基质金属蛋白酶-2(MMP-2)的表达与成釉细胞瘤(AB)临床生物学行为的关系及相关性。方法应用免疫组化EliVisionTM plus法检测69例AB(原发45例,复发24例)、6例成釉细胞癌和16例牙源性角化囊性瘤(KCOT)中RECK、MMP-2蛋白的表达,同时采用RT-PCR方法检测22例AB(原发12例,复发10例)、2例成釉细胞癌和16例KCOT中RECK、MMP-2mRNA的表达水平。所有数据采用SPSS13.0统计软件包进行统计学分析。结果RECK蛋白的阳性表达率在KCOT、AB和成釉细胞癌中依次明显降低(P<0.05),且复发AB显著低于原发AB(P<0.01);AB和成釉细胞癌的MMP-2蛋白阳性表达率均显著高于KCOT(P<0.05);RECK蛋白与MMP-2蛋白在AB中的表达呈负相关(r=-0.431,P<0.001)。RECKmRNA在AB、KCOT中均见表达,但在AB的表达较KCOT显著降低(P<0.001),成釉细胞癌中则无表达;MMP-2mRNA在KCOT、AB和成釉细胞癌中均见表达,但在AB的表达水平较KCOT显著增高(P<0.001),在成釉细胞癌中均呈高水平表达;复发AB的RECK mRNA表达水平较原发AB显著降低(P<0.05),但复发与原发AB的MMP-2mRNA表达之间差异无统计学意义;RECK mRNA与MMP-2mRNA在AB中的表达水平无相关性(P>0.05)。结论RECK表达降低或缺失及MMP-2表达增高与AB的临床生物学行为密切相关。RECK可能通过转录后水平调控MMP-2参与AB的侵润、复发和恶性转化过程。
Objective To explore the relationship and correlation between RECK and matrix metalloproteinase-2 （MMP-2） expression and the clinnieal biological behavior of ameloblastoma （AB）. Methods EliVisionTM plus 2-step immunocbemistry staining method was employed to detect protein expression of RECK and MMP-2 in the paraffin-embedded specimens of 69 cases of AB （including 45 cases of primary AB and 24 cases of recurrent AB）, 6 cases of ameloblastie carcinoma and 16 cases of keratocystic odontogenic tumor （KCOT）, and mRMA expression of RECK and MMP-2 in fresh specimens of 22 cases of AB （including 12 cases of primary AB and 10 cases of recurrent AB）, 2 cases of ameloblastic carcinoma and 16 cases of KCOT was detected using reverse transcription-polymerase chain reaction （RT-PCR）. All data were analyzed by SPSS13.0 software package. Results Immunoreactivity for RECK sequentially reduced in KCOT, AB and ameloblstic carcinoma （P 〈 0.05）, and its expression showed down-regulation in recurrent AB compared with primary AB （P 〈 0.01 ）. Immunoreactivity for MMP-2 in AB and ameloblastic carcinoma enhanced compared with KCOT （P 〈 0.05）. RECK had a negative relationship with MMP-2 in protein expression in AB （r=-0.431）. The expression of RECK mRNA in AB reduced compared with KCOT （P 〈 0.001）, and there was no expression in ameloblsic carcinoma. The mRNA level of MMP-2 in AB increased compared with KCOT （P 〈 0.001）, and there was a high level of expression in ameloblastic carcinoma. RECK mRNA in recurrent AB decreased compared with primary AB （P 〈 0.05）, but MMP-2 mRNA showed no siginificant difference between recurrent AB and primary AB. RECK had no relationship with MMP-2 in mRNA expression in ameloblastoma. Conclusions Decreased or absent expression of RECK and increased expression of MMP-2 may be associated with the clinnical biological behavior of AB, and RECK may participate in the invasion, recurrence and malignant transformation of AB by regulating MMP-2 at post-transcriptional level.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2009年第4期21-25,共5页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
广东省自然科学基金(06021272)
