摘要
利用热水抽提从云芝菌丝体中提取胞内多糖 ,初步纯化后 ,用DEAE SepharoseCL 6B进行分离 ,从中分离出两个带电荷多糖组分即CVP Ⅰ和CVP Ⅱ ,对这两个组分分别用SepharoseCL 6B凝胶柱层析进行纯度鉴定 ,均出现单一峰 ,然后用紫外扫描发现这两个组分均出现蛋白多糖的特征吸收 。
The intracellular polysaccharide was extracted by hot water from the mycelium of coriolus versicolor. After primary purification,CVP Ⅰ and CVP Ⅱ were isolated and purified by DEAE Sepharose CL 6B.Their homogeneity were confirmed by chromatography on Sepharose CL 6B.By means of UV spectrum,it was determined that the polysaccharides belong to proteoglycan.
出处
《天然产物研究与开发》
CAS
CSCD
2003年第1期29-31,共3页
Natural Product Research and Development
基金
江苏省应用基础项目 2 0 0 0 2 6
