摘要
以培矮 6 4S 南京 11F1 和N5 8S FL2 35F1 及F2 代为材料 ,在武汉夏季 (7～ 8月 )自然长日下进行了日平均温度为低温 (2 4℃ )、中温 (2 7℃ )和高温 (30℃ )处理 ,研究了两类不同恢复基因表达的温度敏感性差异。结果表明 ,在自然长日高温和中温条件下 ,其F1 代平均自然结实率均高于 6 8 75 % ,南京 11可完全恢复培矮 6 4S的光敏核不育性。在自然长日高温下 ,FL2 35不能完全恢复N5 8S的光敏核不育性 ,其F1 代平均自然结实率为 2 1 93%～ 2 6 75 %。在自然中温条件下 ,其F1 代平均自然结实率为 4 6 36 %～ 4 8 38% ,FL2 35可以恢复N5 8S的光敏核不育性。进一步分析表明 ,温度仅影响培矮 6 4S 南京F2 育性分布方式和南京 11光敏核不育恢复基因表达程度 ,并不影响恢复育基因的遗传方式。而FL2 35恢复基因表达对高温敏感 ,其临界温度约为 2 7℃ 。
On the basis of the fertility observation of hybrids from Pei'ai 64S and Nongken 58S crossed with an indica variety Nanjing11 and japonica marker line FL235,respectively,The plant growth chambers were employed to expose the F 2 plant individuals to such different day mean temperature as 24℃,27℃ and 30℃ during natural long hour daylight from July to August in Wuhan(30°27′N),with a view to analyzing the difference in the temperature sensitivity of fertility between the two kinds of different fertility restoring genes for two years running.The results showed that,whether it was under long daylight and high temperature or under long daylight and middle temperature,the mean natural seed set percent of F 1 was higher than 68 75%,suggesting that Nanjing11 could completely restore the fertility of Pei'ai 64S.And however,under natural high temperature condition,N58S×FL235 F 1 could set seed naturally with 21 93%～26 75%,the mean natural seed set percent of F 1 was 46 36%～48 38% in natural middle temperature condition,ability of FL235 to restore the fertility of N58S was affected by high temperature.Further analysis proved that temperature could not alter the inheritance mode of F 2 but affect the extent of fertility genes expression in Nanjing11.On the other hand,the expression of fertility genes of FL235 was sensitive to high temperature,whose the putative critical temperature was 27℃,and high temperature influenced not only the genetic interactions but also the segregation modes in F 2 generations.
基金
国家 8 63高技术项目 (863Z16-0 2 -0 2 -0 2 )~~
