摘要
分别以几丁质和胶态几丁质为唯一碳源对木霉菌(Trichodermaspp.)进行液体发酵培养,发酵上清液与胶态几丁质混合,37℃恒温水浴30min,加入3,5 二硝基水杨酸试剂(DNS),沸水浴10min;通过液体的颜色变化检测是否存在还原糖,进而确定木霉产几丁质酶特性.采用此种方法筛选产几丁质酶木霉菌株,结果更为直观、准确.
A trusty and intuitionistic method for screening chitinase produced by Trichoderma spp. was developed. 38 isolates of Trichoderma spp. were cultured in liquid medium with chitin or colloidal chitin as the sole carbon source for 4 days. The supernatant of the fermented broth was mixed with colloidal chitin and heated in waterbath at 37℃ for 30 minute, then 3,5dinitrosalicylic acid reagent (DNS) was added to the mixture, and let them react for 10 min in waterbath. According to the different colour of the mixture, the isolates of Trichoderma spp. which can produce chitinase could be screened.
出处
《仲恺农业技术学院学报》
2003年第1期19-22,共4页
Journal of Zhongkai Agrotechnical College
