摘要
用丙烯酰胺凝胶电泳技术和 3,5_二硝基水杨酸 (DNS)测定还原糖法 ,对不同萌发时间农垦 58F、农垦 58S和农垦 58种子胚中α_淀粉酶同工酶及其酶活性变化进行研究。 0～ 36小时萌发过程中 ,农垦58F和农垦 58Sα_淀粉酶活性高于农垦 58。胚中检测出五条酶。A2、A4和A5为上述种子共有酶带。A3为农垦 58F和农垦 58S特异性酶带。A1为农垦 58特异性酶带。据此认为α_淀粉酶对水稻种子萌发速度起重要调控作用。A1和A3可能是调控种子发芽快慢的重要酶分子。
The change of α_amylase activity and isoenzyme in embryos of Nongken 58F, Nongken 58S and Nongken 58 were analyzed by assay of reducing sugars with 3,5_dinitrosalicylic acid(DNS) and acrylamide gel electrophoresis technique under the different germinating time. The α_amylase activity in the embryos of both Nongken 58F and Nongken 58S were higher than that of Nongken 58 from 0 to 36 h germinating time. Rice seed embryos had 5 isoenzyme bands(A1,A2,A3,A4,A5). A2,A4 and A5 were present in the embryos of Nongken 58F, Nongken 58S and Nongken 58. A3 was specific for both Nongken 58F and Nongken 58S. A1 was specific for the embryos of Nongken 58. These results suggested a higher α_amylase activity be necessary for promoting fast germination of rice seeds, α_amylase isoenzyme A1 and A3 may play an important role in regulation of germination speed.
出处
《植物学通报》
CSCD
北大核心
2001年第2期226-230,共5页
Chinese Bulletin of Botany
基金
中国科学院生物技术与生物科学特别支持费! (STZ98_2_1 4 )资助
关键词
水稻
种子萌发
Α-淀粉酶
萌发速率
Rice, Seed germination, Germinating potential,α_amylase
