摘要
目的 了解苏州地区婴幼儿轮状病毒腹泻的分子流行病学特征。方法 采用酶联免疫吸附试验、巢式聚合酶链式反应对该院 2 0 0 1～ 2 0 0 2年收集的婴幼儿腹泻标本进行轮状病毒检测及血清型研究 ,同时填写统一制定的调查表 ,分析流行特征。结果 775份婴幼儿腹泻标本轮状病毒阳性率为 35 .35 %,血清型分型发现 G3型为流行优势株 ,占 4 9.0 %;其次为 G1型占 32 .9%;再次为 G2、G4 ;未定型占 12 .0 %;G9型有 3例 ,为 1a以内患儿。 G1和 G3全年季节性分布与轮状病毒阳性的分布一致。结论 苏州地区 2 0 0 1～ 2 0 0 2年婴幼儿轮状病毒腹泻的主要血清型为 G3和 G1。
Objective To study the prevalent status of infant rotavirus (RV) diarrhea in Suzhou children's hospital so as to provide basic databases for implementing RV immunization programs. Methods Stool samples of infant diarrhea from Suzhou children's hospital in 2001～2002 were collected and detected with the method of enzyme linked immunosorbent assay and nested reverse transcription polymerase chain reaction. At the same time filled out united questionary and analyzed prevalent character. Results Of the 775 samples detected, the positive rate were 35.35%. G3 (49.0%) was the most prevalent strain followed by G1 (32.9%), G2, G4 and G9 by RV serotype. 30 (12.0%) strains remained non typeable and three strains were G9. The high prevalent age was 12～36 months. The distribution of G1 and G3 was in accord with that of RV positive. Conclusions RV was the most important etiologic agent of infant diarrhea in 2001～2002 in Suzhou, and the predominant serotype was G3 and G1.
出处
《疾病控制杂志》
2003年第2期123-125,共3页
Chinese Journal of Disease Control and Prevention
基金
国际疫苗研究所组织并资助的亚洲轮状病毒监测项目之一 (项目代码 :86 2 5 0 0 10 0 8)
东南大学科学基金资助项目 (编号 :XJ0 190 0 1)
关键词
轮状病毒
致病力
腹泻
婴儿
流行病学
rotavirus/pathogenicity
diarrhea, infantile/epidemiology
