摘要
本文用聚丙烯酰胺凝胶电泳(PAGE)法,于1985～1987年秋末冬初在石家庄与邢台地区对308名急性腹泻患儿粪便标本进行检查,有114份轮状病毒(HRV)阳性,阳性率37.01％。将病毒RNA屯泳后分型,有42份(36.84％)HRV属亚群Ⅰ(短型),72份(63.16％)属亚群Ⅱ(长型)。两个亚群在上述两地区同时存在,但石家庄地区以亚群Ⅱ占优势,邢台地区则以亚群Ⅰ占优势。住院患儿HRV阳性率比门诊患儿高出近4倍,7～24月年龄组患儿所占例数最高,男女之比为1.5:1。感染亚群Ⅱ患儿发热者较多,而感染亚群Ⅰ患儿呕吐者多。黄色稀便的HRV检出率明显高于其它颜色与性状的粪便,而两亚群轮状病毒在粪便颜色与性状分布上无明显差异。
Between the end of autumn and the beginning of winter in 1985～1987, stool specimens of the children with acute diarrhea (308 cases) were investigated by polyacrylamide gel electrophoresis in Shijiazhuang and Xingtai areas. 114(37.01％) of these samples were human rotavirus (HRV) positive. The viruses predominantly infected children with the age of 7-24 months. HRV positive rate of the hospitalized children was four times higher than that of clinic patients. 42(36.84％) specimens were identified of containing strains of subgroup Ⅰ (short patterns), and 72 (63.16％) specimens were HRV subgroup Ⅱ (long patterns). Both subgroups of the virus existed in the two regions. It was found that subgroup Ⅱ rotavirus was a major type in Shijiazhuang area, while subgroup Ⅰ was the predominant one in Xingtai area. No significant differences were observed between the two subgroups in age or sex distribution. But fever was more frequently seen in children infected with HRV subgroup Ⅱ than those of subgroup Ⅰ. Vomiting was more often seen in children infected with rotavirus subgroup Ⅰ. A higher positive rate of HRV was detected in the yellow and watery stool specimens. No significant differences were found between the subgroups in color or properties of stool specimens.
关键词
轮状病毒
腹泻
幼儿
流行病学
rotavirus
subgroups Ⅰ & Ⅱ
molecular & clinical epidemiology
