摘要
收集了北京、邢台、长春、汕头四个地区１９９１年秋冬季节婴幼儿腹泻患儿粪便标本共５５０份。用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）检测到Ａ组轮状病毒３３４例，阳性率为６０．７％。用３２Ｐ标记的各型特异性ＶＰ４和ＶＰ７基因ｃＤＮＡ探针，通过改进的Ｎｏｒｔｈｅｒｎ印迹杂交方法，确定了其中２５０例阳性标本的ＶＰ４、ＶＰ７型别。结果表明我国上述地区１９９１年秋冬季流行的轮状病毒的主要型别为ＶＰ４１Ａ、ＶＰ７１型（６６．４％）和ＶＰ４１Ｂ、ＶＰ７２型（２９．２％），ＶＰ４１Ａ、ＶＰ７３型标本仅在北京地区发现。各种型别毒株之间的混合感染占２．８％。本研究首次报告了北京及我国部分地区婴幼儿腹泻轮状病毒ＶＰ４、ＶＰ７型别及其组合，为我国轮状病毒感染提供了分子流行病学资料。
tool specimens were collected form children with diarrhea in the autumn and winter of 1991 form Beijing ,ChangchunmXingtai and Shantou,located in the north,northeast and south of China.out of 5540 specimens collected,334 (60.7% ) were positive for rotaviruses as deter-mined by polyacrylamide gel electrophoresis (PAGE).VP4 and VP7 genotypes of the viruseswere identified by Northern blot hybridization analysis using 32P labeled VP4 and VP7genotype specific cDNA clones as probes.The data indicated that most of the rotavirusescirculating in this country belong to the genotype combination of VP4 1A and VP7Thirty percent of the virusens belong to VP4 1B and VP7 2.Viruses with VP4 1A and VP7 3 were only found in specimens from Beijing.Rotavirus with VP7 9 was detected from one stool specimen collected in Beijing.Coinfections by rotaviruses with different VP4 and VP7genotypes were identified by Northern blot hybridization as well as by nested polymerase chainreaction(PCR).
出处
《病毒学报》
CAS
CSCD
北大核心
1994年第2期136-144,共9页
Chinese Journal of Virology
关键词
轮状病毒
婴幼儿
腹泻
分型
Rotavirus, Infantile diarrhea, VP4,VP7 genotype,Northern blot hybridization
