摘要
[目的]探讨中药通痹灵对类风湿关节炎软骨破坏的对抗作用。[方法]采用大鼠胶原诱导性关节炎(CIA)模型,用关节软骨免疫组化染色方法,评价造模后通痹灵早期给药治疗对大鼠软骨病变中肿瘤坏死因子—α(TNF—α)、转化生长因子—β(TGF—β)的表达的影响,并与模型组、甲氨碟呤(MTX)组、雷公藤组等进行半定量的平行对比研究。[结果]正常组的软骨组织中TNF—α阳性软骨细胞数呈低表达,且染色浅,与模型组的高阳性表达深染色有显著性差异(P<0.01),也明显优于MTX低剂量组(P<0.01)。通痹灵高、低剂量组及雷公藤高剂量组较接近正常组的表现,优于MTX高、低剂量组(P<0.05),与模型组比较差异有显著性(P<0.01)。正常组软骨组织中TGF—β呈较活跃的表达,模型组与雷公藤高、低组则表达少而染色浅,与正常组比较有显著性差异(P<0.05)。通痹灵高、低剂量组是各病理组中最接近正常组表现的,与模型组比较有显著性差异(P<0.01)。[结论]通痹灵治疗和改善软骨病变的机制可能与其降低软骨细胞异常升高的致炎、致溶骨的细胞因子TNF—α的分泌并升高保护性因子TGF—β的含量有关。
[Objective] To observe the effects of Tongbiling (TBL) prescription in counteracting cartilage destruction in rats with rheumatoid arthritis (RA). [Methods] Collagen-induced arthritis (CIA) rat models were established to observe the therapeutic effects of TBL. After a short-term treatment, expressions of tumor necrosis factor (TNF - a) and transforming growth factor (TGF- P) in cartilage tissue of model group, methotrenxate (MTX) tablets group and Radix Tripterygii Wilfordii (RTW) preparation group were examined by immunohistochemical semi-quantitative assessment method. [ Results ] The number of chondrocytes with positive TNF - a expression was lower and the chondrocytes were stained lighter in normal group than those in model group and low-dose MTX group ( P < 0.01) . Expression of TNF - a in high-dose and low-dose TBL groups and high-dose RTW group was similar to that in normal group and more obvious than that in high-dose and low-dose MTX group ( P < 0.05) and model group ( P < 0.01). Expression of TGF - (3 normal group was more obvious than that in high-dose and low-dose RTW groups ( P < 0.05). Expressions of TNF - a and TGF - (3 in high-dose and low-dose TBL groups were similar to those in normal group and differed from those in model group ( P < 0.01). [Conclusion] TBL can relieve the symptoms of RA and its therapeutic mechanism may be related to the decrease of TNF - a secretion, which can induce the inflammation and the destruction of cartilage , and to the increase of TGF - p level.
出处
《广州中医药大学学报》
CAS
2003年第1期8-12,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金重点项目资助(编号:39930230)
关键词
类风湿关节炎
中药疗法
通痹灵
药理学
桂枝芍药知母汤
软骨
关节
药物作用
肿瘤坏死因子
转化生长因子Β
大鼠
ARTHRITIS, RHEUMATOID/TCD therapy
TONGBILING/phannacology
GUIZHI SHAOYAO ZHIMU DECOCTION/pharmacology
CARTILAGE, ARTICULAR/drug effects
TUMOR NECROSIS FACTOR
TRANSFORMING GROWTH FACTOR BETA
