摘要
目的 :对体外培育牛黄的诱变性进行研究。 方法 :采用Ames试验、小鼠骨髓嗜多染红细胞微核试验、人外周血淋巴细胞体外培养染色体畸变试验等方法进行检测。 结果 :①Ames试验在1250、5000、10000μg/皿的浓度范围内 ,各浓度组MR值在加代谢活化剂S9 和不加代谢活化剂S9 两种情况时均未超过2 ;②微核试验在50、150、500mg/kg3个剂量水平 ,各剂量组的微核率均数及PCE/NCE比值与空白对照组比较均未见显著性差异(P>0.05) ;③人外周血淋巴细胞体外培养染色体畸变试验在样品浓度为1、10、100μg/ml(不加代谢活化剂 ,-S9)和1、10 μg/ml(加代谢活化剂 ,+S9)两种情况下 ,各处理组测得的细胞畸变率与空白对照组比较均无统计学意义(P>0.05)。 结论 :体外培育牛黄在所给剂量范围进行的3种诱变性试验结果均为阴性。
Purpose: To study the mutagenicity of Cultured Calculus Bovis. Methods: The studies were conducted with Ames test, micronuclei test and in vitro chromosome aberration test on human lymphocyte. Results: ① The results of Ames test showed no mutagenic effects with the concentration ranged from 1 250 μg / plate to 10 000 μg / plate. ② In micronuclei test, the mice orally received 50, 150, 500 mg / kg respectively per day for 2 days, no mutagenic effects were observed; and no significant reduction was observed on PCE / NCE ratio, compared with that of the negative control group(P>0.05). ③ The in vitro chromosome aberration test on human lymphocyte showed a negative result with the sample concentration ranged from 1 μg / ml to 100 μg / ml. Conclusion: No mutagenicity was found with Cultured Calculus Bovis in this experimentation.[
出处
《癌变·畸变·突变》
CAS
CSCD
2003年第1期35-37,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
体外培育牛黄
诱变性
染色体畸变
Cultured Calculus Bovis
Mutagenicity
Chromosome aberration
