摘要
选用AEV(VR株 )CEF适应毒制备抗原 ,免疫Balb/c小鼠。应用杂交瘤技术以间接ELISA方法筛选获得一株分泌抗AEV(VR)单克隆抗体的杂交瘤细胞。制备细胞上清及腹水进行单抗特性鉴定 ,经染色体计数、免疫球蛋白类及亚类测定、特异性试验、稳定性试验、SDS PAGE电泳 ,表明该杂交瘤细胞染色体数目介于 90～ 110之间 ,该单克隆抗体亚类为IgG2a,分子量为 142KD ,ELISA测定细胞上清效价为 1∶1× 10 4 ,腹水效价为 1∶1× 10 6,与其它病原无交叉反应 ,抗体分泌稳定。
A hybridoma cell line was obtained which can secret monoclonal antibodies against Avian Encephalomyelitis(AEV).The characterization of monoclonal antibodies was performed by chronosomal counting,specificity test,stability test and SDS-PAGE electrophoresis.The results showed that the chromosomal numbers were between 90 and 110,the subclass was IgG 2a,the titer of supernatant was 1∶1×104,the titer of ascitic fluid was 1∶1×106,the molecular weight was 142 KDa,and the secreting of monoclonal antibodies was stable,and no cross reacticity was observed between the antibodies and the other avain viruses(NDV,EDSV,PMV 2,IBVH120?491?M 41,AIV).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2003年第2期191-194,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省农科院青年基金支持项目 ( 980 410 )
关键词
禽脑脊髓炎
单克隆抗体
制备
鉴定
Avian Encephalomyelitis
Monoclonal
Antibody
Identification
