摘要
实验采用称量法和气相色谱法分别测定了性早熟前后中华绒螯蟹的肝胰腺指数(HI)、肝脂含量(LC)和肝胰腺脂肪酸组成。雌雄蟹的HI和LC在早熟前后的变化趋势一致,都是早熟个体低于未成熟个体。在性早熟前,雌雄蟹的肝胰腺脂肪酸组成基本一致,只是雌蟹中C20∶5n-3较高(P<0.05)。然而,对早熟前后的个体作比较时,发现雌雄蟹变化情况有很大差异:(1)早熟雌蟹中C16∶1n-7、C16∶0和C18∶0高于未成熟雌蟹(P<0.01),而C20∶5n-3、C20∶4n-6和C22∶6n-3则低于后者(P<0.01);(2)早熟雄蟹中C18∶1高于未成熟雄蟹(P<0.05),而C14∶0(P<0.05)、C16∶1n-7(P<0.05)和C20∶5n-3(P<0.01)皆低于后者。以上结果表明:雌雄蟹在性早熟前后存在肝胰腺脂肪酸组成变化的差异,且C20∶5n-3可能主要参与成熟蜕壳和膜的构建,而C22∶6n-3可能主要参与卵巢发育和卵黄的发生。
The hepatopancreas index(HI), lipid content(LC) and fatty acid composition in Eriocheir sinensis before and after the precocity, were compared by gravimetry and gas chromatography. The results showed that HI and lipid content both decreased after the precocious molt, regardless of their sex. As for fatty acid composition of hepatopancreas, immature male and female individuals were very similar, except for C20∶5n-3 significantly higher in the latter (P<0.05). However, it varied with the sex when the precocious and immature crabs were compared. In female, the percentage content of C16∶1n-7, C16∶0 and C18∶0 were significantly higher in precocious individuals, but that of C20∶4n-6, C20∶5n-3(EPA) and C22∶6n-3(DHA) significantly lower(P<0.01). And in male, there was only C18∶1 significantly higher (P<0.05) in precocious individuals while C14∶0 (P<0.05), C16∶1n-7 (P<0.05) and C20∶5n-3 (P<0.01) lower. According to the results, it might come to the conclusions that EPA is very important during precocious molt and construction of membranes, while DHA mainly participates in the development of ovary and vitellogenesis.
出处
《水产学报》
CAS
CSCD
北大核心
2003年第1期57-61,共5页
Journal of Fisheries of China
基金
上海市教育委员会重点学科项目
关键词
中华绒螯蟹
性早熟
肝胰腺
肝胰腺指数
肝脂含量
脂肪酸组成
Eriocheir sinensis
precocity
hepatopancreas
hepatopancreas index
lipid content
fatty acid composition
