摘要
探明MRSA感染的血清学特点及质粒分布 ,为其预防和治疗提供科学依据。做血浆凝固抑制试验—中和法和质粒DNA提取用碱裂解法。结果血浆凝固酶Ⅱ型的检出阳性率为 5 4.0 % ( 47/ 87) ,Ⅳ型为 2 7.5 % ( 2 4/ 87) ,其余为Ⅶ和Ⅲ型。按菌株来源分析 :病房工作人员和住院患者的MRSA以Ⅱ型居多 ,Ⅳ型次之 ,有明显的偏重集中趋势 ,而门诊患者MRSA血浆凝固酶型别分布散乱。多数菌株有质粒 ,分布复杂。表明沈阳地区流行的主要血浆凝固酶型别为Ⅱ型和Ⅳ型。
For prophylaxis and therapy with scince evidence,We illustrate serum coagulase type of MRSA(Methicillin-resistant staphylo coccus aureus)infection and the plasmid distribution by serum inhibition test-NA method and plasmid DNAextration-alkaline lysis.The positive rate of coagulas type Ⅱ is 54.0%(47 versus 87)and coagulase type Ⅳ is 27.5%(24 versus 87),the others are type Ⅶ and type Ⅲ.Analysis according to the resourse of strains:Coagulas type Ⅱ is the highest ratio in the sickroom workmen and in-hospital patients,the second-rate is type Ⅳ and the trend concentrate significantly.While in the clinical patients,the distribution of the typing is dispersal.Most of the strains have plasmid and distribute complex.The result suggest the major type prevalence in Shenyang are coagulase type Ⅱ and type Ⅳ.;
出处
《微生物学杂志》
CAS
CSCD
2003年第1期22-23,共2页
Journal of Microbiology
