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荧光聚合酶链反应检测在耐甲氧西林金黄色葡萄球菌检测中的应用 被引量：1

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摘要 目的探讨荧光聚合酶链反应(PCR)检测在耐甲氧西林金黄色葡萄球菌(MRSA)检测中的应用。方法收集上海市监狱总医院肺部感染患者的痰液标本,共3768份,分别进行PCR、纸片扩散抗菌试验、琼脂稀释法检测。结果采用一般细菌培养分离鉴定与荧光PCR检测均检出103例金黄色葡萄球菌,阳性率2.7%。采用纸片扩散抗菌试验检测,检出57株MRSA阳性;采用琼脂稀释法检测,检出58株MRSA阳性。采用荧光PCR检测,有58株MecA基因扩增呈阳性。结论荧光PCR检测具有快速、简易、稳定、敏感性强、特异性高的特点,可用于MRSA的快速鉴定。
作者 瞿伟华 陈碧英 郎立中
机构地区 上海市监狱总医院检验科 上海市第七人民医院检验科
出处 《国际检验医学杂志》 CAS 2013年第9期1130-1132,共3页 International Journal of Laboratory Medicine
关键词 葡萄球菌 金黄色 甲氧西林抗药性 聚合酶链反应 纸片扩散抗菌试验 琼脂稀释法
分类号 R440 [医药卫生—诊断学]
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国际检验医学杂志
2013年 第9期

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