摘要
目的 :探讨肝素结合表皮生长因子样生长因子 (HB EGF)在皮肤鳞状细胞癌中的作用机制 .方法 :用原位杂交的方法检测正常组织、癌旁组织和鳞状细胞癌皮损组织中HB EGFmRNA的表达 .结果 :在正常皮肤组织中 ,HB EGF表达于基底层 (10 0 0 % ) ,基底上层仅有少量表达 (12 5 % ) ;在癌旁组织中 ,HB EGF不仅位于基底层 (10 0 0 % ) ,且在基底上层也有表达 (91 7% ) ;在鳞状细胞癌皮损中 ,全层几乎无HB EGF的表达 (8 3% ) .结论 :HB EGF在鳞状细胞癌发病的早期阶段起关键作用 ,基底层中的HB EGF对保持正常表皮形态有重要作用 .
AIM: To investigate the pathogenesis of HB EGF in skin squamous cell carcinoma. METHODS: HB EGF mRNA in normal tissues, tissues adjacent to cancer and skin squamous cell carcinoma lesions were detected by hybridization in situ . RESULTS: In normal skin tissues, the stain of HB EGF was located in the basal layer of epidermis (100 0%) and only little expression in superabasal layers (12 5%). In tissues adjacent to cancer, there was expression not only in the basal layer (100 0%) but also in superabasal layers (91 7%), while in squamous cell carcinoma, there was nearly no expression (8 3%).CONCLUSION: HB EGF may play an important role in the early pathogenesis of skin squamous cell carcinoma and play the role of maintaining the phenotype of normal skin tissues in basal layer.
出处
《第四军医大学学报》
CAS
北大核心
2003年第4期366-368,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (30 0 70 699)
关键词
皮肤鳞状细胞癌
HB-EGF
皮肤肿瘤
原位杂交
肝素
heparin
epidermal growth factor urogastrone
skin neoplasms
carcinoma,squamous cell
in situ hybridization
