摘要
目的 研究皮肤干细胞在全层皮肤创面愈合过程中的分布与增殖分化特征 ,以及该特征在创面愈合过程中的作用。方法 2 0只Wistar大鼠背部各制备 4个面积为 2 5 4cm2 的全层皮肤缺损创面 ,将创面随机分为 2组 ,即银锌霜治疗组 ( 40个创面 ) ,空白对照组 ( 40个创面 )。分别于伤后 3天、1周、2周和 3周以组织学检查动态观察各组治疗效果 ,并以 β1整合素、角蛋白 19(K19)免疫组化法检测表皮干细胞在创面愈合过程中的分布情况。结果 两组创面愈合率在银锌霜组为 80 % ( 32 /40 ) ,对照组为 6 0 % ( 2 4/ 40 )。两组创面肉芽组织于各时相点均未见 β1整合素、K19阳性细胞出现 ,但于创缘表皮的棘层或颗粒层出现了散在的 β1整合素和K19同时染色阳性细胞 ,且越接近创面这些阳性细胞更加密集 ,组织学上与基底层的阳性细胞无直接联系 ;其数量随着创面的缩小渐渐增加 ,直到创面愈合。创面上皮化后 ,这些阳性细胞逐渐减少 ,并随着愈合创面表皮脚的出现而消失 ;而感染创面的阳性细胞数量明显少于未感染创面。结论 表皮干细胞能动的参与创面的修复 ,创缘表皮干细胞再分布的主要功能可能是促进创面再上皮化。
Objective To study the redistribution of epidermal stem cells in regenerating wound tissues, and to elucidate the role of epidermal stem cells during wound repair. Methods 80 circular full-thickness wounds were produced on both sides of the back in 20 male Wistar rats (4 wounds in each animal). Then the 80 wounds were randomly divided into 2 groups: group A (treated with sulfadiazine silver- sulfadiazine zinc cream, n=40) and group B (without any treatment, n=40). The infection and healing process of the wounds were observed with naked eyes. Five mice were killed one time 3, 7, 14, and 21 days after the wounding. Two wounds with surrounding normal skin tissues were collected from each mouse. Routine histological examination was conducted with HE staining. β 1 integrin and keratin19 (K19), markers of epidermal stem cells, were employed to determine the distribution of epidermal stem cells with streptavidin-peroxidase(SP)immunohistochemical method. Results The healing rate of wounds was 80%(32/40)in group A,and 60%(24/40) in group B. No β 1 integrin and K19 positive cells was found in the granulation tissue of all wounds in these 2 groups at any time point during the healing process. However, a few β1 integrin and K19 positive cells,bearing no anatomic relation with the epidermal stem cells in the basal layer,were found scattering in the stratum spinosum and stratum granulosum of the epidermis on the wound edges. The closer to the wound the more concentrated the β 1 integrin and keratin19 positive cells. Their numbers increased gradually along with the shrinking of the wound surface until the wound completely healed. The numbers of the β 1 integrin and keratin19 positive cells decreased gradually after epithelialization. The numbers of the β 1 integrin and keratin19 positive cells were obviously lower in the infected wounds than in the uninfected wounds. Conclusion Epidermal stem cells participated in the healing of wound. The function of redistribution of epidermal stem cells in wound edges seems to promote the re-epithelialization of granulating wounds.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第3期228-231,共4页
National Medical Journal of China
基金
国家重点基础研究发展规划资助项目(G19990 5 42 0 4)
国家杰出青年科学基金资助项目 ( 395 2 5 0 2 74)
国家自然科学基金资助项目 ( 30 2 30 370
30 170 96 6 )
