摘要
目的 观察不同厚度异体皮制备的微粒皮混合自体微粒皮移植于大鼠背部全层皮肤缺损后对创面愈合的影响。 方法 制作大鼠全层皮肤缺损创面模型。以移植面积扩张比为 5∶1的自体微粒皮为对照组 ( 10只 ) ,两种不同厚度异体皮制备的微粒皮与同样扩张比的自体微粒皮混合移植为实验组 ,其中实验 1组异体微粒皮厚度为 0 .3mm(10只 ) ,实验 2组 0 .6mm(6只 )。比较移植后 2、3、4周 3组大鼠创面愈合率、收缩率及组织学差异。 结果 创面愈合率 :移植后 2周实验 1组大鼠( 94 .5 8± 3.99) %和实验 2组 ( 95 .2 8± 1.93) %均高于对照组 ( 88.2 8± 6 .85 ) % (P <0.0 5 ),移植后 3周实验 2组 ( 94 .5 5± 3.4 7) %高于实验 1组 ( 89.5 1± 4 70 ) %及对照组 ( 88.5 1± 5 .5 9) % (P <0.0 5),移植后 4周 3组比较 ,差异无显著性意义 (P >0 0 5 )。创面收缩率 :实验 1组大鼠与对照组比较 ,差异无显著性意义 (P >0.0 5),实验 2组各时相点均低于实验 1组及对照组 (P <0.0 5)。组织学检查 :移植后 2周实验组大鼠有明显的淋巴细胞灶性浸润 ,移植后 4周 3组之间比较 ,差异无显著性意义 ( P>0.0 5 )。结论 适量的异体微粒皮混合自体微粒皮移植 ,可以促进创面愈合 ;混合移植等量异体微粒皮时增加其真皮厚度 。
Objective To observe the influence of mixed grafting of autologous and allogeneic microskin on burn wound healing. Methods Autologous microskin grafting (expansion rate 5∶1) was employed as control.Autologous microskin mixed with the allogeneic microskin with the thickness of 0.3mm and 0.6mm,respectively, were designated as experimental groups 1 and 2 (EP1 and EP2).The wound healing rate, wound contraction rate,and histological changes were observed on the 2nd, 3rd and 4th weeks after the grafting. Results The wound healing rate in two experimental groups (94.58±3.99)% in EP1,and (95.28±1.93)% in EP2 was significantly higher than that in the control group (88.28±6.85)% at the end of the 2nd week after the grafting(P<0.05)The wound healing rate in experimental group 2(94 55±3.47)% was obviously higher than that in control (88.51±5.59)% and experimental group 1(89.51±4 70)% at the end of the 3rd week after grafting(P<0.05). There was no obvious difference in wound healing rate among the three groups at the end of the 4th week after grafting.Obvious lymphocytic infiltration was observed by histological examination between epidermis and dermis in the two experimental groups at the end of the 2nd week after grafting. But there was no obvious difference among the three groups 4 weeks after grafting. Conclusion The wound healing could be improved by mixed skin grafting with appropriate quantity of allogeneic and autologous microskin. Furthermore, the wound contraction could be ameliorated if the thickness of allogeneic dermis was increased in the mixed grafting even with the same proportion of allogeneic to autologous microskin.
出处
《中华烧伤杂志》
CAS
CSCD
2004年第4期229-231,共3页
Chinese Journal of Burns
基金
第三军医大学回国人员启动基金资助项目(2 0 0 2 0 9)
西南医院科技创新基金资助项目 (2 0 0 2 0 1)
