摘要
背景与目的:表皮生长因子(epidermalgrowthfactor,EGF)及其受体(epidermalgrowthfactorreceptor,EGFR)在肿瘤形成过程中起着重要作用,胆结石胆囊炎与胆囊癌关系密切,本研究通过观察EGF、EGFR在慢性胆囊炎、胆囊癌中的表达,探讨两者与胆囊癌发生的关系。方法:采用免疫组化SABC法检测手术切除的41例胆囊癌、26例单纯增生、14例不典型增生和10例正常胆囊组织中EGF、EGFR及增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)的表达。结果:EGF、EGFR阳性表达率在胆囊癌(63.4%、70.7%)、不典型增生(71.4%、85.7%)中分别高于单纯增生(15.4%、27%)和正常胆囊组织(0%、0%)(P<0.01);PCNA评分有随胆囊粘膜病变程度的加重而递增的趋势,在正常胆囊组织、单纯增生、不典型增生和胆囊癌中分别为1.0、1.0、1.9288±0.9972和3.0488±0.669,(P<0.01);EGF、EGFR表达与PCNA的表达有统计学意义(P<0.05),EGF、EGFR、PCNA的表达与胆囊癌TNM分期无统计学意义(P>0.05)。结论:EGF、EGFR的表达参与胆囊癌的发生,与细胞增殖活性相关。
BACKGROUND &OBJECTIVE:Epidermal growth factor(EGF) and its receptor(EGFR) play an important role in tumorigenesis. Cholelithiasis and cholecystitis were closely related to gallbladder carcinoma.This study was designed to investigate the relationship between the expression of EGF and EGFR in chronic cholecystitis and gallbladder carcinoma. METHOD:SABC immunohistochemistry was used to determine the expression of EGF, EGFR, and proliferating cell nuclear antigen (PCNA) in surgically resected specimens of 41 gallbladder carcinomas, 26 simple hyperplasia tissues, 14 dysplasia tissues,and 10 normal gallbladder tissues. RESULTS:The positive expression rates of EGF and EGFR were higher in gallbladder carcinomas(63 4%,70 7%) and dysplasia tissues (71 4%,85 7%) than in simple hyperplasia tissues (15 4%,27%) and normal gallbladder tissues (0%,0%)(P< 0 01); PCNA counting score tended to increase with the severity of mucosa change degree(P< 0 01), which were 1 0 in normal tissues, 1 0 in simple hyperplasia tissues, 1 9288±0 9972 in dysplasia tissues,and 3 0488±0 669 in gallbladder carcinomas. Significantly positive correlation were shown between EGF,EGFR, and PCNA(P< 0 05). There was no correlation between EGF, EFGR, and PCNA expression and tumor TNM stage(P >0 05).CONCLUSION:Expression of EGF and EGFR is involved in the gallbladder carcinogenesis, and is related to high activity of cell proliferation.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2003年第3期262-265,共4页
Chinese Journal of Cancer
关键词
胆囊肿瘤
EGF
EGFR
PCNA
免疫组织化学
Gallbladder carcinoma
Epidermal growth factor (EGF)
Epidermal growth factor receptor (EGFR)
Proliferating cell nuclear antigen (PCNA)
Immunohistochemistry
