摘要
目的 研究TNFα激活的星形胶质细胞在慢性癫痫复发中的作用。 方法 分别用反相高效液相色谱法和免疫组织化学反应检则细胞释放谷氨酸 (Glu)和NF κBp6 5表达的变化。将TNFα激活的星形胶质细胞条件培养液 (ACM)注射入慢性马桑内酯致痫发作间期大鼠之侧脑室 ,观察动物行为和脑电图的变化 ,并用免疫组织化学反应观察海马NF κBp6 5和胶质原纤维酸性蛋白 (GFAP)表达的变化。 结果 1 TNFα可明显促进星形胶质细胞释放Glu ,并快速诱导星形胶质细胞核内NF κBp6 5的表达 ;2 侧脑室注射ACM可引起大鼠 4级癫痫行为及典型的痫样脑电图表现 ;注射ACM后 0 5h即可观察到海马回特别是CA1区细胞核内p6 5的表达 ,2～ 4h达高峰 ,8h恢复至对照水平 ;注射ACM后 1h海马GFAP免疫反应阳性细胞数开始高于对照组 ,4h达高峰 ,8h仍明显高于对照组。
Objective To investigate the effect of astrocytes activated by TNFα on the relapse of epilepsy. Methods The purified astrocytes of cultured rat hippocampus were stimulated by TNFα.The expression of NF\|κ Bp65 was checked by immunocytochemical method and the release of glutamate(Glu) from astrocytes was measured by high performance liquid chromatography(HPLC).The astrocytes conditioned medium(ACM) stimulated by TNFα was collected and injected into lateral ventricle of chronic Coriaria Lactone kindled rats during the interictal period.The changes both in behavior and electroencephalogram(EEG) were recorded.The expression of NF\|κ Bp65 and glial fibrillary acidic protein(GFAP) of hippocampus were also studied by immunocytochemical method. Results 1.TNFα could promote the release of Glu by astrocytes and quickly induce the nuclear expression of NF\|κ Bp65 in cultured astrocytes.2.After lateral ventricular injection,four class epileptic behaviors were induced,which were confirmed by epileptic charge EEG.At 0\^5?h after injection of ACM,NF κ Bp65 expression in hippocampal cells especially in the CAl area were induced.It reached to the maximal levels at 2\|4?h and returned to control level at 8h.At 1h after injection of ACM,the increase of the number of positive GFAP \|immunoreactivity(IR) cells of hippocampus could be observed.The expression of GFAP reached to the top level at 4h and still maintained at a higher level than the control group until 8h.Conclusion\ Astrocytes activa\|ted by TNFα could induce the relapse of epilepsy by some solube neuro\|active substance.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第1期21-26,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 9970 3 79)
