摘要
目的 探讨急性排斥反应过程中Fractalkine(FKN)及其受体CX3CR1在移植心脏局部表达的意义及环孢素A(CsA)对它们的影响。 方法 施行大鼠异位心脏移植术 ,移植大鼠分为 3组 ,每组 45只 ,对照组 5只。SD大鼠间的移植为同系移植组 (A组 ) ,Wistar至SD大鼠的移植分为未用CsA干预组 (B组 )及CsA干预组 (C组 ) ,健康SD大鼠为对照组。采用RT PCR方法检测排斥反应中移植心脏局部FKNmRNA的表达水平 ,免疫组化方法检测FKN和CX3CR1的蛋白表达水平。 结果FKNmRNA与蛋白在A组各时间点和对照组均呈低水平表达 ;在B组的表达变化与急性排斥反应的进程相关 ,术后第 7天FKNmRNA表达上调至峰值 (0 8± 0 2 6) ;C组应用CsA后 ,FKNmRNA表达峰值显著低于B组 (t=2 3 90 ,P <0 0 5 )。FKN和CX3CR1蛋白分别定位于移植心脏血管内皮细胞及间质浸润的单个核细胞中。 结论 急性排斥反应过程中 ,FKN及其受体CX3CR1表达上调 ,与移植物间质单个核细胞浸润密切相关 ;抑制FKN
Objective To investigate the expression of fractalkine(FKN) and its receptor CX3CR1 in cardiac allografts and the effect of Cyclosporin A(CsA). Methods Three groups of rats underwent heterotopic cardiac transplantation, 45 cases in each group and 5 cases in control group: SD to SD regarded as isograft group (group A), Wistear to SD divided into CsA untreated allograft group (group B) and CsA treated allograft group (group C), normal SD rats as control group. The FKN mRNA expression was detected by one step RT PCR method and the expression of FKN and CX3CR1 protein was detected by standard ABC immunohistochemical technique. Results The expression of FKN mRNA and protein was weak in both isografts and normal heart specimens. The changes of FKN mRNA expression were correlated with the process of acute allograft rejection. The peak of FKN mRNA expression(0 8±0 26) appeared on the seventh day after transplantation, which could be downregulated by CsA significantly( t =2 390, P <0 05). FKN protein was located in endothelia cells and its receptor CX3CR1 was located in infiltrating mononuclear cells in allografts. Conclusions Upregulation of FKN and its receptor was significantly correlated with the trafficking of mononuclear cells which play an important role in acute allograft rejection. It may be one of the important mechanisms of CsA to intervene the acute rejection by inhibiting the activation of the FKN CX3CR1 pathway.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2003年第2期139-142,共4页
Chinese Journal of Surgery
基金
上海市医学领先专业重点学科发展基金资助项目(9830 0 5 )
