摘要
目的观察趋化因子Fractalkine(FKN)在重症急性胰腺炎(SAP)中的表达。方法在大鼠SAP动物模型中,应用ELISA方法检测血清FKN的表达水平,同时应用免疫组化方法检测胰腺组织中FKN的表达;收集40例轻症急性胰腺炎(MAP)和22例SAP患者的血样,应用ELISA方法检测血清FKN表达水平。结果在大鼠SAP模型中,血清FKN浓度明显高于正常对照组(P<0.05),同时,胰腺组织中观察到FKN的高表达;MAP和SAP患者的FKN浓度均高于正常对照组,而且SAP患者显著高于MAP患者(P<0.05)。结论趋化因子FKN作为是预测急性胰腺炎严重程度的有效指标之一,值得进一步深入研究。
Objective To explore the expression of Fractalkine（ FKN） in severe acute pancreatitis（ SAP）. Methods The serum FKN level of rat model in SAP was examined by enzyme- linked immunosorbent assay（ ELISA）. The expression of FKN level was by immunohistochemistry in pancreas tissue. The serum FKN concentration was examined in patients with mild acute pancreatitis（ MAP）（ n = 40）,SAP（ n = 22） and health controls（ n = 26） by ELISA. Results Compared with control group,the level of serum FKN was significantly increased in rats with SAP（ P〈0. 05）. The result of immunohistochemistry showed that FKN was overexpressed in pancreas tissue. Compared with the normal group the concentration of FKN was higher in MAP and SAP patients（ P〈0. 05）. Conclusion FKN may be a valuable marker for SAP assessment.
出处
《宁夏医学杂志》
CAS
2016年第3期207-208,I0001,共3页
Ningxia Medical Journal
基金
国家自然科学基金资助项目(81260086)
