摘要
目的 研究妇科肿瘤患者原发性肿瘤组织细胞凋亡相关基因bcl 2、bax、caspase3的表达及其与mtDNA突变的相关性。方法 取 32例妇科恶性肿瘤组织进行研究 ,应用RT PCR方法检测bcl 2、bax、caspase3基因的表达。采用PCR SS CP及DNA测序检测mtDNA突变。结果 在妇科恶性肿瘤组织中 ,bcl 2 ,bax ,caspase3基因的表达率分别为 78 1%、4 3.8%及 34 4 %。bcl 2与caspase3、bax表达之间呈明显的负相关 ,Caspase3与Bax基因表达的共同阳性率和共同阴性率分别为71 4 %及 94 4 %。在mtDNA突变的 2 2例中 ,2 0例 (90 9% )同时检测到bcl 2的表达 ,Bax及caspase3基因在mtDNA突变组的表达率分别为 2 7 3%和 13 6 % ,明显低于非突变组。结论 在妇科恶性肿瘤组织中存在bcl 2基因的高表达和bax、cas pase3基因的低表达。bcl 2与caspase3、bax表达之间呈明显的负相关 ,caspase3与bax基因存在共表达关系。细胞凋亡相关基因表达与mtDNA突变亦存在一定的相关性。
Objectives: To investigating the gene expression of BCL?2, BAX, and Caspase3 in tumor tissues of gynecologic oncology patients and their relationship to mtDNA mutation. Methods: Total 32 samples of gynecological malignant tumors were taken. RT?PCR were done to examine bcl?2?bax?caspase3 gene expression. PCR?SSCP and DNA sequence were done to detect mtDNA mutation. Results: The rates of bcl?2, bax, and caspase expression in gynecological malignant tumors were 78.1%(25/32),43.8%(14/32),and 34.4%(11/32)respectively. There were negative correlation among bcl?2,caspase3, and bax. The rates of co?positive and negative of caspase3 and bax were 71.4% and 94.4% respectively. In total 22 cases of mtDNA mutation, 20 cases(90.9%) had bcl?2 co?expression. Bax and caspase3 gene expression in the mtDNA mutation group were 27.3% and 13.6% respectively. They were much lower than that of mtDNA non?mutation group. Conclusions: There are high expression of bcl?2 gene and low expression of caspase3 and bax gene in gynecological malignant tumors. The expression of Bcl?2 had a negative relationship with expression of bax and caspase3. But bax and caspase3 have co?expression. There are positive relationship between cell apoptosis associated gene expression and mtDNA mutation.
出处
《中国优生与遗传杂志》
2003年第1期34-36,共3页
Chinese Journal of Birth Health & Heredity
