摘要
目的 研究caspase3在原发性肝细胞癌 (HCC)中的表达并观察其与细胞凋亡的关系。方法 原位杂交检测caspase3在HCC中的表达 ,原位末端标记检测细胞凋亡。结果 39例HCC中的2 1例 (5 3 8% )不同程度的表达caspase3,而 46 2 %的HCC未见caspase3表达。HCC中caspase3阳性率低于癌旁非癌肝组织中的阳性率 (87 5 % ,7/ 8) ,差异有显著性 (P <0 0 5 )。caspase3的表达与肿瘤的分化有关 ,中等至高分化的HCC中阳性率 (72 2 % )明显高于低分化HCC中的阳性率 (38 1% ,P <0 0 5 ) ,而与肿瘤的大小、分级无关。尽管在已转移的HCC中阳性率 (6 2 5 % )高于无转移的HCC中的阳性率 (5 1 6 % ) ,但差异无显著性 (P >0 0 5 )。检测HCC中的细胞凋亡发现 ,肿瘤组织中caspase3的表达与肿瘤细胞的凋亡无明显的关系 ,caspase3阳性的HCC中 ,其细胞凋亡指数 (AI)与caspase3阴性的HCC中的AI相比 ,差异无显著性 (7 1‰∶6 6‰ ,P >0 0 5 )。结论 caspase3失表达在HCC的发生中起重要作用 ,但与HCC中细胞凋亡的关系不很密切。
Objective To investigate the relationship between caspase3 expression and apoptosis in hepatocellular carcinoma (HCC). Methods In situ hybridization was employed to determine caspase3 expression, and in situ end labeling was used to detect apoptotic cells in HCC. Results Twenty one of 39 (53.8%) cases of HCC were found to express caspase3 transcripts, while 46.2% of HCC failed to express it. Adjacent non cancerous liver tissues showed more caspase3 positiveity (87.5%, 7/8) as compared with HCC ( P <0.05). The expression of caspase3 was correlated with HCC differentiation, as 72.2% (13/18) of moderately to well differentiated HCC showed positive caspase3 transcripts, while only 38.1% of poorly differentiated HCC was caspase3 positive ( P <0.05). No relationship was found between caspase3 and tumor size or grade or metastasis, although 62.5% (5/8) of HCC with metastasis was caspase3 positive but it did not differ significantly from that without metastasis ( P >0.05). Expression of caspase3 alone did not affect the apoptosis index (AI) of HCC. AI was 7.1‰ in caspase3 positive tumors ( n =21), as compared to 6.6‰ ( n =18) in caspase3 negative cases ( P > 0.05). Conclusion Although loss of caspase3 expression may contribute to hepato carcinogenesis, its expression may not be related to cell apoptosis in HCC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2000年第1期39-41,共3页
Chinese Journal of Oncology
关键词
肝肿瘤
肝细胞
caspase3基因
基因表达
Liver neoplasms
Carcinoma, hepatocellular
Caspase3 gene
Gene expression
Apoptosis
