摘要
目的 :探讨如何以简便快捷的方法获得大量生长状态良好的高纯度黑色素细胞。方法 :以包皮组织作为细胞来源 ,用消化法获得表皮细胞悬液 ,用本实验室设计的方法对细胞进行纯化 ,然后用两种不同培养基MC1、MC2培养细胞 ,Dopa染色和透射电镜鉴定细胞来源 ,通过MTT和流式细胞仪分析观察细胞的生长状态。结果 :用本实验室的纯化方法获得的黑色素细胞中未见角朊细胞和成纤维细胞污染 ,MTT和流式细胞仪检测结果显示用MC2培养的黑色素细胞处于旺盛的增殖状态 ,明显高于MC1培养的黑色素细胞。结论 :结果表明本实验方法可以获得大量处于良好生长状态的高纯度黑色素细胞。
AIM:To develop a rapid and reproducible method for the isolation of pure melanocytes in good conditions from foreskin. METHODS:The melanocytes were isolated and purified by a method employed in our lab. Two different media MC1 and MC2 were used to culture the cells. The biological conditions of the cells were examined by MTT detection and flowcytometry(FCM) analysis. RESULTS:The melanocytes obtained by this method were free of keratinocytes and fibroblasts contamination. And the cells maintained in MC2 showed better proliferation ability than those cultured in MC1. CONCLUSION:The pure melanocytes in good conditions can be attained rapidly by our method.
出处
《牙体牙髓牙周病学杂志》
CAS
2003年第1期21-23,T002,共4页
Chinese Journal of Conservative Dentistry
