摘要
目的：构建PEGFP-HPV16E6／E7表达载体，观察其在角航细胞中的瞬时表达．方法：基因重组技术，基因转染技术．结果：酶切鉴定证实HPV16E6／E7片段已克隆到pEGFP-N3的EcoRI和BamHI位点之间，在转染角肮细胞中观察到绿色荧光蛋白的表达．结论：pEGFP-HPV16E6／E7表达载体便于观察，转染细胞中HPV16E6／E7-GFP融合蛋白的表达情况及蛋白定位，适用于对HPV16E6／E7分子生物学特性及致瘤机理的研究．
AIM: To construct PEGFP--HPV16E6/E7 expression vector and to detect its transient expression in transfected keratinocytes. METHODS: Techniques of gene recombination and gene transfection were employed. RESULTS: identification of PEGFP HPV16E6/E7 by enzyme digestion showed that HPV16E6/E7 fragment had been cloned into EcoR 1 and BamH I sites of PEGFP N3 and the expression of GFP in transfected keratinocytes was observed. CONCLUSION: pEGFP--HPV16E6/E7 expression vector makes it easyto assess the expression of HPV16E6/E7--GFP fusion proteinand the protein localization in trans feeted cells.
出处
《第四军医大学学报》
1999年第8期659-660,共2页
Journal of the Fourth Military Medical University
基金
国家自然科学基金!39570656
关键词
乳头瘤病毒
表达载体
构建
基因转染
角朊细胞
human papillomavirus
expression vector
construction
gene transfection
