摘要
目的 观察丹参对自发性高血压大鼠左室肥厚的作用 ,探讨其作用机制。方法 18只 8周龄的自发性高血压大鼠随机分成 3组 ,1组于 8周处死 ,另 2组分别经腹腔注射丹参或蒸馏水 ( 1g·kg- 1 ·d- 1 ) ,共 10周。测量大鼠尾动脉收缩压 (SBP)及左心室重量指数 (LVMI)。检测心肌细胞的直径 (TDM)和面积 (CA)、心肌组织胶原容积积分 (CVF)、血管周围胶原面积和管腔面积比例 (PVCA)以及转移生长因子 - β1 (TGF - β1 )的表达。结果 与 8周龄的自发性高血压大鼠相比 ,18周龄大鼠的SBP、LVMI、TDM、CA、CVF和PVCA显著增加 ,TGF - β1 表达上调 ,丹参对自发性高血压大鼠可抑制左室肥厚的发展和心肌组织中TGF - β1 的高表达 ,收缩压亦有明显的降低 (P <0 0 1)。结论 长期应用丹参可预防自发性高血压大鼠左室肥厚的形成 ,其机制可能与丹参降低了心肌细胞TGF - β1 的表达有关。
Objective To observe the effects of Radix Salviac Miltiorrhizae (RSM) on left ventricular hypertrophy (LVH) in spontaneously hypertensive rats (SHR) and to investigate its mechanisms. Methods 18 SHRs aged 8 weeks old were divided into three groups randomly. One group the rats were killed at 8 weeks. RSM or distilled water (1 g·kg -1 ·d -1 ) was injected abdominal cavity into two groups for 10 weeks. Systolic blood pressure (SBP) and left ventricular mass index (LVMI) were measured. HE?VG and immunohistochemistry staining combined with computed morphometry were used to evaluate the diameter (TDM) and cardiomyocyte area(CA), the collagen volume fraction (CVF),perivascular circumferential area (PVCA) and the transforming growth factor-β 1(TGF-β 1) expression in the left ventricular tissue. Results Compared with 8-week-old rats, the SBP,LVMI,TDM,CA,CVF,PCVA and TGF-β 1 expression increased markedly in 18-week-old SHRs. RSM could inhibit the LVH significant and TGF-β 1 expression decreased, however SBP reduced obviously(P<0.01). Conclusion RSM could inhibit the development of LVH in SHR, which was probably related to the decreased expression of cardiac TGF-β 1.
出处
《中国急救医学》
CAS
CSCD
北大核心
2003年第2期71-73,共3页
Chinese Journal of Critical Care Medicine
基金
湖北省自然科学基金资助课题 (No .2 0 0 0J0 6 4)
关键词
丹参
预防
自发性高血压
大鼠
左室肥厚
转移生长因子Β1
Spontaneously hypertensive rat(SHR)
Left ventricular hypertrophy(LVH)
Radix Salviac Miltiorrhizae(RSM)
Transforming growth factor-β1(TGF-β1)
