摘要
目的 通过观察妊娠肝内胆汁淤积症 (ICP)患者胎盘细胞凋亡调控基因p5 3、bax和bcl 2在胎盘中的表达 ,探讨细胞凋亡基因在胎盘细胞凋亡中的作用。方法 采用原位末端标记法(TUNEL)和免疫组织化学方法对 3 1例ICP患者 (ICP组 )的胎盘组织中p5 3、bax和bcl 2基因表达及调亡指数进行检测 ,并以 3 1例正常妊娠妇女的胎盘组织作对照 (对照组 )。结果 (1)ICP组胎盘组织中细胞滋养细胞、合体滋养细胞、蜕膜细胞及间质细胞中细胞凋亡指数分别为 (49 1± 9 1) %、(46 6±9 8) %、(3 5 1± 9 5 ) %、(3 8 7± 9 7) % ,明显高于对照组的 (2 2 5± 6 2 ) %、(2 1 6± 5 2 ) %、(17 9±6 2 ) %、(17 0± 4 7) %。两组比较 ,差异有极显著性 (P <0 0 1)。 (2 )而调控基因p5 3的表达 ,ICP组明显高于对照组 ,两组比较 ,差异有极显著性 (P <0 0 1) ;bax的表达在两组滋养细胞中比较 ,差异无显著性 (P >0 0 5 )。但在合体滋养细胞、蜕膜细胞及间质细胞中 ,ICP组明显高于对照组 ,两组比较 ,差异有极显著性 (P <0 0 1) ;bcl 2的表达在ICP组胎盘组织细胞滋养细胞、合体滋养细胞、蜕膜细胞及间质细胞中 ,均明显低于对照组 (P <0 0 1)。 (3 )p5 3、bax和bcl 2在ICP组合体滋养细胞中的表达分别是 (75 9± 8 2 ) %。
Objective To investigate the changes of gene p53, bax and bcl 2 expression and to study the role on cell apoptosis in placenta through apoptosis in placenta with intrahepatic cholestasis of pregnancy (ICP) Methods TdT mediated dUTP nick end labeling(TUNEL) and immuno histochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index and the expression of p53, bax and bcl 2 in placenta tissues Results The apoptosis index in placental cytotrophoblast, syncytiotrophoblast, decidual cells and mediate cell on ICP group was significantly higher than that in control group( P <0 01) The expression of p53 was higher than those of control Bax expression in syncytoitrophoblast, decidual cell and mediate cell in ICP group was higher( P <0 01), but the expression of bax in placental cytotrophoblast cell was not significantly higher( P >0 05) The expression of bcl 2 of these positions in ICP group was lower than that in control group( P <0 01) p53,bax and bcl 2 expressionsin (±s) % in the syncytoitrophoblast cells of placenta with ICP is 75 9±8 2, 65 8±13 8, 45 3±11 3 Conclusion The placental dysfunction may closely relate to the apoptosis Abnormal overexpression of p53, bax and the low expression of bcl 2 on placental tissue of ICP was the main reason of placental apoptosis
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2003年第1期5-7,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
妊娠肝内胆汁淤积症
胎盘
细胞凋亡
调控基因
研究
Cholestasis,intrahepatic
Pregnancy complications
Placenta
Apoptosis
Gene,p53
Genes,bcl 2
