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电场诱导细胞融合装置的技术参数设计 被引量:3

DETERMINATION OF PERFORMANCE PARAMETERS REQUIRED FOR AN ELECTRIC FIELD——INDUCED CELL FUSION DEVICE
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摘要 电场诱导是80年代以来兴起的一种细胞融合技术,在细胞遗传工程实践中具有很大应用价值。细胞融合仪是进行电场诱导融合的核心装置,对其研制和改进能促进这项新技术的不断完善。根据电场融合基本原理,在考虑细胞性质、融合小室几何参数以及兼顾装置经济实用性等因素的基础上,分析讨论了融合装置所应满足的技术性能参数。其输出交流电场频率应为0.3—4.0 MHz,最大峰峰值应达到50 V;脉冲时程为1—100μs,最大幅度应达到500V;输出阻抗应小于0.4 kΩ。 The electric field-induced cell fusion is a new technique for somatic hybridization since 1980's, and has great application potential in cell genetic engineering. Electric field-generating unit is the core device for this technique. Taking such factors as cell character, geometric parameters of fusion chamber and device practicality into consideration, this paper determined the desirable performance parameters required for an electricfusion device to meet the wide-ranging use in cell fusion practice.
机构地区 东北林业大学
出处 《东北林业大学学报》 CAS CSCD 北大核心 1992年第6期15-21,共7页 Journal of Northeast Forestry University
基金 国家教委资助优秀年轻教师基金
关键词 细胞融合 电场 脉冲电路 参数 Celt fusion Electric fields Pulse circuits Parameters
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参考文献4

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