摘要
细胞融合仪是进行细胞融合研究的核心装置。作者研制出一种性能参数较好而造价低廉的电诱导细胞融合仪。其输出交流电场频率及电压峰峰值分别为０．５－４．０ＭＨｚ和０－７０Ｖ；脉冲时程和幅度分别为４－１００μｓ和０－５００Ｖ；带负载能力大于几种国内外产品。用本装置对香菇和丝核菌原生质体进行了融合诱导，对电场诸参数与融合效果之间的关系进行了探讨。结果表明本装置性能稳定，使用效果不次于进口日本产品，可用于真菌、细菌或植物的细胞融合研究。
Electric field-generating device is essencially important for conducting somatic hy-bridization by electro-induced cell fusion. The author designed a low·cost electric cell fusion de vice,the electric parameters of which are as follows:frequency and peak-to-peak voltage of AC field being 0.5-4.0MHz and 0-70V respectively; pulse width and intensity being 4-100μs and 50-500V respectively. Fusion tests were successfully conducted using this device with protoplas-ts from fungi Lentinus edodes and Rhizoctonia solani.The performance features of the deive as compared with several domestic and imported products were discussed.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第1期14-17,共4页
Microbiology China
基金
国家教委优秀年轻教师基金
