摘要
目的 克隆小鼠共刺激分子B7-1基因,测序并构建其真核表达载体。方法 从小鼠脾组织中提取总RNA.,用RT-PCR方法将B7-1的DNA扩增出,克隆到真核表达载体pcDNA3中,测定其cDNA序列。结果 测序结果表明我们克隆的B7-1基因序列与CenBank中序列有1个碱基不同,所编码的氨基酸发生突变。结论 已成功构建小鼠B7-1的真核表达载体,为进一步应用B7-1进行肿瘤基因治疗打下基础。
Objective To clone, sequence and construct eukaryotic expression vector of mouse B7-1 gene. Methods The total RNA was isolated from mouse spleen.The B7-1 cDNA was synthesized and amplied from the mRNA by RT-PCR and the PCR product was cloned into pcDNAS eukaryotic expression vector and subjected to DNA sequencing. Results Sequencing analysis revealed that the amino acid sequence of mouse B7-1 had one variation in nucleotide sequence which caused one amino acid residue displace( compared with that of published sequence). Conclusion We successfully construct the eukaryotic expression vector of mouse B7-1 gene, which provide a solid basis for the study of B7-1 on antitumor genetherapy.
出处
《哈尔滨医科大学学报》
CAS
2002年第6期428-430,共3页
Journal of Harbin Medical University
基金
国家自然科学基金资助项目(30170916
30070739)
黑龙江省自然科学基金资助项目(D01-04)
