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表达小鼠B7-1基因的重组逆转录病毒载体构建及真核表达 被引量：1

Construction of recombinant retroviral vectors expressing B7-1 gene in cell
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摘要 目的:构建表达小鼠B7-1基因的重组逆转录病毒载体并在真核细胞中检测其蛋白表达。方法:PCR法获基因,定向克隆连接到逆转录病毒载体上,得到重组逆转录病毒载体,酶切法鉴定,真核细胞中检测蛋白表达。结果:酶切所得片段与所需相符,在真核细胞中检测到蛋白表达。结论:成功构建重组的逆转录病毒载体,为今后研究奠定了基础。 Objectiue： To construct recombinant retroviral vectors expressing B7-1 gene. Methods： PCR was performed with special primers of B7-1 to get B7-1 gene. The cDNA fragment of B7-1 was ligated into retroviral vectors. Then it was identified by enzyme digestion and detected its protein expression in cell. Results： The amplified fragment was 930bp with RT-PCR In addition, special protein was gotten, Conclusion： Recombinant retroviral vectorsexpressing B7-1 gene and protein should provide a foundation for the application of B7-1 gene in further study.
作者 刘为军 王昆华 欧阳一鸣 张杰 王廷华 龚昆梅 包维民 张勇学 钟鸣 凌平 黄映光 张剑 朱宇
机构地区 云南省第一人民医院普外科 昆明医学院分子生物学研究所
出处 《中国现代普通外科进展》 CAS 2007年第1期18-20,共3页 Chinese Journal of Current Advances in General Surgery
基金 云南省第一人民医院科研基金资助项目
关键词 基因 B7—1 基因重排 基因表达调控 基因转移技术 Genes,BT-1 .Gene rearrangement .Gene expression regulation .Gene transfer techniques
分类号 Q782 [生物学—分子生物学]
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参考文献6

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共引文献5

同被引文献6

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中国现代普通外科进展
2007年 第1期

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