摘要
将从各地收集到的 2 4个灵芝菌株进行深层发酵培养 ,通过比较菌丝生长速度和发酵生物量 ,筛选出 8个优良菌株。根据菌株间拮抗反应及蛋白酶同工酶分析结果 ,确定 8个菌株中的G2和G15、G33和G2 7为同一菌株 ,属同株异名。进一步比较 8个菌株的菌丝发酵生物量 ,胞内、胞外多糖含量等指标 ,确认菌丝发酵生物量、胞内多糖含量较高的灵芝 0
Ganoderma lucidum strains with higher biomass and higher mycelium growth rate in submerged culture were screened out from 24 G. lucidum strains collected. The result of antagonistic test and proteinase isozyme test showed that G2 and G15, G33 and G27 were two groups of synonym strains. Further test of submerged culture verified that the polysaccharide content and mycelial biomass of G.lucidum strain 0770 were relatively high among 8 G.lucidum strains screened out. And G. lucidum strain 0770 was considered to be the optimum strain for submerged culture.
出处
《食用菌学报》
2002年第4期18-21,共4页
Acta Edulis Fungi
基金
上海市农委"抑瘤灵芝多糖双高灵芝菌株的筛选"(2 0 0 1第 3- 14号 )的部分内容
关键词
深层发酵
灵芝
高产菌株
筛选
菌丝生物量
多糖含量
Ganaderma lucidum
Submerged culture
Mycelial biomass
Strain screening
Polysaccharide content
