摘要
[目的 ]探讨雌激素受体拮抗剂在非小细胞肺癌治疗中的作用。 [方法 ]用逆转录多聚酶链式反应 (RT-PCR)测定肺腺癌细胞株 SPC- A- 1的雌激素受体 m RNA(ER- m RNA)表达 ,分别在去激素培养条件下观察不同浓度的他莫昔芬 (Tam)、4-羟基他莫昔芬 (OHTam)和雌二醇 (E2 )对其生长速度、细胞周期时相、ER- m RNA水平和细胞形态学的影响。 [结果 ] SPC- A- 1肺腺癌细胞株有 ER- m RNA表达 ,低浓度 E2 (1 0 -8mol/ L)可促进其生长 ,Tam(1 0 -6mol/ L )和 OHTam(1 0 -6mol/ L)可抑制 E2 (1 0 -8mol/ L )的生长刺激作用。去激素环境下细胞生长明显减慢 ,在无激素环境中亦能抑制细胞生长 ,抑制发生在 G0 / G1 期 ,并有细胞内质网扩张等结构改变 ,ER- m RNA表达水平下降。 [结论 ]肺腺癌 SPC- A- 1细胞株的生长具有雌激素依赖性 ,应用 ER拮抗剂 Tam和 OHTam能抑制其生长。肺癌细胞中
To investigate the effects of estrogen antagnists in the treatment of non small lung cancer (NSCLC). RT PCR was applied to the detection of ER mRNA expressed in human lung cancer cell strain, SPC A 1. DCC(Dextran treated with charcoal) treated fetal bovine serum was used for hormone free culture medium. Estrodiol, tamoxifen(Tam) and 4 hydroxytamoxifen(OHTam) were added respectively in different concentrations into the cultrure medium. Cell numbers, Cell cycles, levels of ER mRNA and morphological changes in the above culture mediums were examined. There was expressed ER mRNA in SPC A 1. Estrogen stimulated the growth of the cell below the concentration of 10 7 mol/L. In estrogen stripped medium, the growth of the cells was significantly hampered and both Tam(10 6 mol/L) and OHTam(10 6 mol/L) inhibited the growth of SPC A 1 no matter whether estrodiol was added or not, which occurred in G 0/G 1 phase. Simultaneously changes of endoplasmic reticulum and mitochondria in ultra structure of SPC A 1 and declining of the levels of ER mRNA in SPC A 1cell lines were noticed. [CONCLUSION] The growth of SPC A 1 depends on estrogen. Tam and OHTam can inhibit the growth of SPC A 1 in estrogen dependent manner as well as estrogen independent manner. ER levels in human lung cancer cell lines can be auto regulated.
出处
《药学进展》
CAS
2002年第6期344-348,共5页
Progress in Pharmaceutical Sciences
基金
江苏省科委社会发展基金资助项目 (BS97365)
