摘要
目的:探讨他莫昔芬(TAM)对人肝癌SMMC7721细胞增殖的影响以及与容积激活性氯电流的关系。方法:MTT法检测TAM对细胞增殖的作用;流式细胞术检测TAM对细胞周期的影响;全细胞膜片钳技术观察TAM对容积激活性氯电流的抑制作用。结果:TAM能浓度依赖性地抑制SMMC7721细胞的增殖和低渗激活的容积激活性氯电流,两者呈正向直线相关;TAM也阻滞细胞周期于G0/G1期。结论:TAM通过调节容积激活性氯通道的开放抑制SMMC7721细胞的增殖,容积激活性氯通道可能是TAM抗肿瘤特别是雌激素受体阴性肿瘤的靶点。
Objective： To investigate the role of the volume-activated chloride current （Icl,vol）on inhibition effects of tamoxifen on the proliferation in human hepatocellular carcinoma cells SMMC 7721. Methods： MTT assay was used to determine the inhibition of TAM on cell proliferation. The whole cell patch clamp technique was used to observe the effects of tamoxifen on Icl,vol. Results： Tamoxifen inhibited the cell proliferation, Icl,vol in a concentration-dependent manner and arrested cell cycle on G0/G1 phase. Analysis of the relationships between the current and cell proliferation showed that the current was positively correlated with cell proliferation. Condusion： These data indicate that tamoxifen may inhibit cell proliferation by modulating Icl,vol. The Icl,vol may be an extra-estrogen receptor site of action for tamoxifen and may account, in part, for its anti-growth effects on estrogen receptor-negative cancers.
出处
《中国医药导报》
CAS
2009年第19期39-41,共3页
China Medical Herald
基金
国家自然科学基金(30800435)
广东省医学科研基金(B2008086)
关键词
肝癌
他莫昔芬
细胞增殖
氯通道
Hepatocellular carcinoma
Tamoxifen
Cell proliferation
Chloride channels
