摘要
目的:研究硫酸软骨素A(chondroitin sulfate-A,CS-A)对成骨细胞生长发育及矿化的影响。方法:将人胚成骨细胞放入含有不同浓度的CS-A的DEMA培养液中培养,检测成骨细胞的增殖情况、碱性磷酸酶活性及细胞层钙含量。结果:低浓度(1 mg/ml和0.5 mg/ml)CS-A能促进成骨细胞的增殖,高浓度(10 mg/ml)CS-A对成骨细胞增殖有轻度抑制作用。培养基中游离的CS-A能减少细胞层中的钙含量,高浓度时显著地抑制了钙在细胞层的聚集;CS-A对成骨细胞碱性磷酸酶的活性无抑制作用。结论:CS-A对成骨细胞的生长增殖有一定的影响,CS-A在体内可调节成骨细胞矿化,但不影响成骨细胞的分化。
Objective;The purpose of this study was to investigate the effects of chondroitin sulfate-A (CS-A) on culture of osteoblasts in vitro.Methods: Various concentrations of CS-A were added to the cultures of human osteoblasts, and the cell proliferation quantity, calcium content and alkaline phosphatase activity of cell layers were evaluated. Results: The CS-A in low concentrations (1 mg/ml and 0.5 mg/ml) promoted the proliferation of osteoblast, while in high concentration(10 mg/ml )inhibited the proliferation of osteoblast slightly. The CS-A prevented calcium accumulation in the cell layers, although CS-A had no inhibition on alkaline phosphatase activity. Condusion: The CS-A can affect proliferation and mineralization of osteoblast, but has no effect on differentiation of osteoblasts.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2002年第6期411-413,425,共4页
West China Journal of Stomatology
