摘要
以鸡胸软骨为原料,采用稀碱-双酶(胰蛋白酶和胃蛋白酶)相结合的方法提取硫酸软骨素。研究了胃蛋白酶水解硫酸软骨素的影响因素,结果显示,胃蛋白酶水解部分pH值为5.9,酶解时间为100 m in,料液比为1 000∶1.1(g/mL),酶解温度为40℃,在此条件下,硫酸软骨素产率达到25.80%,纯度为85.65%,质量有较大提高。
The method of dilute alkali-enzyme hydrolysis to extract chondroitin sulfate from the chicken breast cartilage was studied. The effect of stomach proteinase on the production purity of the CS was studied. The results showed the best reaction conditions ： pH value of stomach proteinase 5.9, enzymolysis time 100 min,ratio of filter liquor to enzyme 1 000 ： 1.1 （ g/mL）, enzymolysis temperature 40 ℃. Under the conditions, the highest production rate was 25.80% and production purity was 85.65%, its quality was improved better.
出处
《应用化工》
CAS
CSCD
2009年第12期1766-1768,共3页
Applied Chemical Industry
关键词
硫酸软骨素
胃蛋白酶
纯度
chondroitin sulfate
stomach proteinase
purity
