摘要
建立了动物性食品肉和蛋中氨基甲酸酯类农药 (NMCs)多组分残留高效液相色谱紫外检测 (HPLC-UV)分析方法。采用C1 8柱 ,甲醇 水 (6 0 :40 )为流动相 ,于 2 10nm进行检测。样品以丙酮 氯化钠 二氯甲烷一步法提取和分配 ,提取液浓缩后 (浓缩步骤须控制温度不超过 40℃ ) ,经过两次交联聚苯乙烯凝胶 (Bio BeadsS X3 )净化 ,收集流分为 5 0～ 80ml。 5种NMCs检测限为 3 2～ 13 3μg kg ,标准曲线线性良好 ,(r=0 991～ 0 997)。 5种NMCs标准平均过柱回收率为 89 8%～ 10 9 7% ,本方法净化效果良好。蛋和肉样中添加两种不同浓度的NMCs ,平均回收率分别为 81 6 %～ 97 2 %和 70 7%～ 94 0 %。
A high-performance liquid chromatographic(HPLC) method using a reverse phase column(C 18)and methanol-water(60:40)mobile phase with ultraviolet detector (210nm) was established for simultaneous determination of 5 carbamate insecticides (aldicarb,MTMC,carbofuran,carbaryl and isoprocarb).The extraction and partition were combined into one step by saturating the extract with NaCl and driving the water away by dichloromethane.It was cleaned up twice by gel permeation(Bio-Beads S-X 3).The linearity for NMCs standards was good(r=0.991-0.997)and the detection limits of 5 carbamate insecticides were 3.2-13.3μg/kg.The recovery fortified with two levels of standard was in the range of 81.6% to 97.2% and 70.7% to 94.0% respectively.
出处
《卫生研究》
CAS
CSCD
北大核心
2002年第6期465-467,共3页
Journal of Hygiene Research
