摘要
目的 观察慢性乙型肝炎 (CH B)患者血清、肝组织中HBVDNA含量与庚型肝炎病毒(HGV)感染的关系 ,探讨HGV感染对CH B患者乙型肝炎病毒 (HBV)复制的影响。方法 应用逆转录 聚合酶链反应 (RT PCR)、免疫组织化学法、荧光定量PCR(FQ PCR)技术方法对 5 6份CH B患者血清HGVRNA、肝组织HGVAg、血清及肝组织中HBVDNA含量分别进行了检测 ,并将血清HGVRNA与肝组织HGVAg的表达、HGVRNA、HGVAg阳性与阴性患者HBVDNA含量分别进行了对比研究。结果 血清HGVRNA、肝组织HGVAg阳性分别为 10份 (17.9% )、8份 (14 .3% )。血清HGVRNA阳性与肝组织HGVAg表达显著相关 (P <0 .0 1) ,但部分肝组织HGVAg阴性患者亦有血清HGVRNA表达。血清HGVRNA、肝组织HGVAg阳性与阴性患者血清及肝组织中HBVDNA含量差异无显著性 (P >0 .0 5 )。结论 HGV感染对CH B患者HBV复制无影响。HGV可在肝脏中复制 ,但致病性可能较微弱。
Objective To observe the relationship between the amount of HBV DNA in serum/liver tissue and HGV infection in patients with chronic hepatitis B (CH B) for exploring the effect of HGV infection on hepatitis B virus(HBV) replication of CH B.Methods HGV RNA in serum,HGV nonstructural region 5 (NS5) antigen (HGV Ag) in liver tissue and the amount of HBV DNA in serum,liver tissue were detected for 56 patients with CH B by reverse transcription polymerase chain reaction (RT PCR) assay,peroxidase antiperoxidase (PAP) immunohistochemical method and fluorescence quantitative PCR assay, respectively. Then the relationship between HGV Ag expression in liver tissue and HGV RNA expression in serum was analysed and the amount of HBV DNA in serum and liver tissues from the serum HGV RNA or liver tissue HGV Ag positive patients were compared with those of the serum HGV RNA or liver tissue HGV Ag negative patients, respectively. Results Ten (17.9%) and eight (14.3%) patients were positive for serum and liver tissues,respectively.HGV RNA expression in serum was closely related to HGV Ag expression in liver tissues, but there was HGV RNA in serum from some of the liver tissues HGV Ag negative patients (cases of HGV RNA and HGV Ag positive or negative,HGV RNA positive but HGV Ag negative, HGV RNA negative but HGV Ag positive, respectively: 5,43,5,3,( P <0.01). There was no significant difference in the amount of HBV DNA in serum and liver tissues between HGV RNA or HGV Ag positive and negative patients ( P >0.05). Conclusion HGV infection may not affect HBV replication. Liver is the site of HGV replication, but HGV probably also replicates in extrahepatic tissues. HGV hepatic pathogenicity is probably mild and further studies are still needed.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2002年第4期326-328,共3页
Chinese Journal of Experimental and Clinical Virology
