摘要
目的 探讨庚型肝炎病毒 (HGV)感染对慢性乙型肝炎 (CH B)患者乙型肝炎病毒 (HBV)复制的影响。方法 应用逆转录 聚合酶链反应 (RT PCR)、过氧化物酶与抗过氧化物酶复合物 (PAP)法免疫组织化学、荧光定量PCR(FQ PCR)技术对 5 6例CH B患者血清HGV RNA、肝组织HGV Ag、血清及肝组织中HBV DNA含量分别进行了检测 ,并将血清HGV RNA与肝组织HGV Ag的表达、HGV RNA ,HGV Ag阳性与阴性患者HBV DNA含量进行了对比研究。 结果 血清HGV RNA、肝组织HGV Ag阳性分别为 8例 (1 4 3 % )、1 0例 (1 7 9% )。血清HGV RNA阳性与肝组织HGV Ag表达显著相关 (P <0 .0 1 ) ,但部分肝组织HGV Ag阴性患者亦有血清HGV RNA表达。血清HGV RNA、肝组织HGV Ag阳性与阴性患者血清及肝组织中HBV DNA含量均无显著性差异 (P >0 .0 5 )。结论 HGV感染对CH B患者HBV复制无影响。肝脏是HGV的复制场所 ,但可能亦有肝外组织器官中复制。HGV至多具有微弱的致病性 ,但仍然需要进一步研究。
Objective To explore the effect of hepatitis G virus (HGV) infection on hepatitis B virus (HBV) replication of patients with chronic hepatitis B (CH-B). Methods HGV-RNA in serum, HGV antigen (HGV-Ag) in liver tissues and the amount of HBV-DNA in serum, in liver tissues were detected for 56 patients diagnosed as CH-B by liver biopsy by reverse transcription-polymerase chain reaction (RT-PCR) assay, peroxidase antiperoxidase (PAP) immunohistochemical staining assay and fluorescence quantitative PCR assay, respectively. Then the relationship between HGV-Ag expression in liver tissues and HGV-RNA expression in serum was analysed and the amount of HBV-DNA in serum and liver tissues from the serum HGV-RNA or liver tissues HGV-Ag positive patients were compared those of the serum HGV-RNA or liver tissues HGV-Ag negative patients, respectively. Results 10(17.9%), 8(14.3%)patients were positive for serum and liver tissues, respectively. HGV-RNA expression in serum was significantly releated to HGV-Ag expression in liver tissues (P<0.01), but some of the liver tissues HGV-Ag negative patients also expressed serum HGV-RNA. There were no significant difference in the amount of HBV-DNA in serum and liver tissues between HGV-RNA or HGV-Ag positive and negative patients (P>0.05). Conclusion HGV infection does not affect HBV replication. Liver is the site of HGV replication, but HGV also replicates in extrahepatic tissues probably. HGV hepatic pathogenicity is at most mild, but further studies still need to be performed.
出处
《临床军医杂志》
CAS
2001年第3期60-64,共5页
Clinical Journal of Medical Officers
关键词
庚型肝炎病毒
乙型肝炎病毒
复制
肝组织
定量聚合酶链反应
hepatitis G virus
hepatitis B virus
replication
liver tissue
quantative polymerase chain reaction
