摘要
目的了解山东地区不同人群中SEN病毒(SENV)感染的状况与其致病性。方法应用逆转录聚合酶链反应法(RT-PCR)检测了2545份不同人群和肝病患者血清中SENVDNA,并对17例不明原因ALT增高,单独SENVDNA阳性的非甲—非戊型肝炎患者行肝活检。结果SENVDNA总阳性检出率为20.87%(53/254),其中非甲—非戊型肝炎血清检出率最高为59.57%(28/47),明显高于健康献血员3.23%(1/31)、非肝病患者5.45%(3/55)、输血后丙型肝炎42.86%(12/48)、血液透析患者15.38%(2/13)和慢性乙肝患者8.75%(7/80),SENV在各种人群中存在着显著性差异(P<0.01)。SENV感染其主要病理学改变为汇管区炎症,淋巴细胞集聚,碎小坏死,小叶内病变较轻,呈散在和点状坏死,而桥样坏死,脂肪性变较少。结论山东地区不同人群及肝病患者中存在着较高SENV感染率;SENV感染可能具有嗜肝性;而且可能与肝功损害有关,是引起非甲—非戊型肝炎重要病原,输血后丙肝和有血液透析史患者SENV感染率较高,提示对献血员还应严格筛选。
Objective To know the infective status and pathogenesis of SEN virus in different population in Shandong province.Methods SENV DNA were amplified and detected by RT-PCR from 254 sera of patients in different population in Shandong province;17 non A-E hepatitis patients with increased ATL level but SENV DNA positive received the liver biopsies.Results SENV DNA positive rate was totall 20.8%(53/254).Among these population,the population of non A-E hepatitis had the highest SENV DNA positive rate, 59.57%(28/47).However,in the population of health blood donor the positive rate was 3.23%(1/31)and that of non-liver diseases was 5.45%(3/55).The difference was significant statistically.The SENV DNA positive rate in the population of post-transfusion hepatitis C, 42.86%(12/48),was higher than 15.38%(2/13)in hemodialysis patients and 8.75%(7/80)in chronic hepatitis B.There were significant higher differences among different population(P<0.01).The main lesions were periportial inflammation,dense lymphoid aggregates and piecemeal necrosis.Intralobular injury of hepatocytes was rather mild which taken on scattered soptty necrosis;bridging necrosis and fatty degeneration were not common respectively.Conclusion The SENV infection rate is high in different population in Shandong province.SENV infection might be hepatotroic,probably it is correlated with the lesin of the liver and it's the important pathogen of non A-E hepatitis. The post-transfusion hepatitis C and hemodialysis patients have the higher SENV infection rate,which suggest that blood donors biopsies should be strictly screened.
出处
《实用医药杂志》
2004年第3期254-257,共4页
Practical Journal of Medicine & Pharmacy
关键词
SEN病毒
肝病
聚合酶链反应
肝活检
组织病理学
SEN virus Liver diseases Polymerase chain reaction Liver biopciesHistopathology
